ABCA1 Antibody
(Synonyms: TGD; ABC1; CERP; ABC-1; HDLDT1; HPALP1; HDLCQTL13)ABCA1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to ABCA1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Antibody Dilution Buffer (for Western Blot)
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Antibody Dilution Buffer (for Immunohistochemistry, IHC)
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Antibody Dilution Buffer (for Immunostaining)
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WB/IP Lysis Buffer
HY-K1000
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GelHP Protein Gel (Tris-Gly, 10%, 10 well)
HY-K1111
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BCA Protein Assay Kit
HY-K2002
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Ultra High Sensitivity ECL Kit
HY-K1005
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Ponceau Staining Solution (Ready-to-use, 1×)
HY-K1109
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1,000 |
Product Details
ABCA1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to ABCA1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 254 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 254 kDa
Recombinant protein within human ABCA1 aa 1,101-1,312.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ABCA1 catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP. Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs. Transports preferentially phosphatidylcholine over phosphatidylserine. May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs. Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain[1][2][3][4].
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Subcellular Localization
Cell membrane; Endosome
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Expression
Tissue_Specificity: Widely expressed, but most abundant in macrophages.
Induction: By bacterial lipopolysaccharides (LPS). LPS regulates expression through a liver X receptor (LXR) -independent mechanism. Repressed by ZNF202. -
Isoforms & Post-Translational Modification
ABCA1 has an amino acid length of 2261, molecular weight is 254302 Da.Ser-205 位点的磷酸化调控磷脂外排ABCA1 的氨基酸个数为 2261 个，分子量为 254302 Da。Phosphorylation on Ser-2054 regulates phospholipid efflux
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Subunit
Interacts with MEGF10.
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SwissProt ID
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Synonyms
TGD; ABC1; CERP; ABC-1; HDLDT1; HPALP1; HDLCQTL13
Documentation
[1]. Quazi F, et al. Differential phospholipid substrates and directional transport by ATP-binding cassette proteins ABCA1, ABCA7, and ABCA4 and disease-causing mutants. J Biol Chem. 2013 Nov 29;288(48):34414-26. [Content Brief]
[2]. Segrest JP, et al. ABCA1 is an extracellular phospholipid translocase. Nat Commun. 2022 Aug 16;13(1):4812. [Content Brief]
[3]. Krimbou L, et al. Molecular interactions between apoE and ABCA1: impact on apoE lipidation. J Lipid Res. 2004 May;45(5):839-48. [Content Brief]
[4]. Marcil M, et al. Mutations in the ABC1 gene in familial HDL deficiency with defective cholesterol efflux. Lancet. 1999 Oct 16;354(9187):1341-6. [Content Brief]
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