RACGAP1 Antibody (YA9637)
(Synonyms: CYK4; GAP; Gap1; ID GAP; MgcRacGAP; Racgap1)RACGAP1 Antibody (YA9637) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RACGAP1.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
|---|---|
| Dilution Ratio | 1:1000 |
Product Details
RACGAP1 Antibody (YA9637) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RACGAP1.
-
Host Rabbit
-
Clonality Recombinant,Monoclonal
-
Species ReactivityHuman, Mouse, Rat
-
Observed Molecular WeightObserved band size: 71 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 71 kDa
Synthetic peptide within human RACGAP1 aa 51-100 / 632.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
RACGAP1 is a Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Sequentially binds to ECT2 and RAB11FIP3 which regulates cleavage furrow ingression and abscission during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Has a critical role in erythropoiesis. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells[1][2][3].
-
Subcellular Localization
Nucleus; Cytoplasm; Cytoplasm, cytoskeleton, spindle; Cytoplasmic vesicle, secretory vesicle, acrosome; Cleavage furrow; Midbody, Midbody ring; Cell membrane; Midbody
-
Expression
Tissue_Specificity: Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.
Induction: Expression is down-regulated during macrophage differention of HL-60 cells. -
Isoforms & Post-Translational Modification
RACGAP1 has an amino acid length of 632, molecular weight is 71027 Da.
Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation. Phosphorylation on Ser-164 plays a major role in mediating interaction with ECT2. Phosphorylation on Ser-157 does not appear to contribute to binding to ECT2 -
Subunit
Heterotetramer of two molecules each of RACGAP1 and KIF23.
-
SwissProt ID
-
Synonyms
CYK4; GAP; Gap1; ID GAP; MgcRacGAP; Racgap1
-
Research Field
Cell Biology
Documentation
[1]. Simon GC, et al. Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis. EMBO J. 2008 Jul 9;27(13):1791-803. [Content Brief]
[2]. Kawashima T, et al. MgcRacGAP is involved in the control of growth and differentiation of hematopoietic cells. Blood. 2000 Sep 15;96(6):2116-24. [Content Brief]
[3]. Wontakal SN, et al. RACGAP1 variants in a sporadic case of CDA III implicate the dysfunction of centralspindlin as the basis of the disease. Blood. 2022 Mar 3;139(9):1413-1418. [Content Brief]
Powered by Bioz
