RACGAP1 - Rac GTPase activating protein 1 Gene

Also Known as CYK4; CDAN3B; ID-GAP; HsCYK-4; MgcRacGAP

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 29127

About RACGAP1

Cytogenetic location: 12q13.12 Genomic coordinates (GRCh38): 12:49,989,162-50,033,440 (from NCBI)

This gene has 28 transcripts (splice variants) and 269 orthologues. Broad expression in testis (RPKM 17.3), bone marrow (RPKM 8.3) and 20 other tissues.

Summary

This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]

RACGAP1 Products (12)

mRNA	Protein	Name
NM_001126103.3	NP_001119575.1	rac GTPase-activating protein 1 isoform a
NM_001126104.3	NP_001119576.1	rac GTPase-activating protein 1 isoform a
NM_001319999.2	NP_001306928.1	rac GTPase-activating protein 1 isoform a
NM_001320000.2	NP_001306929.1	rac GTPase-activating protein 1 isoform a
NM_001320001.2	NP_001306930.1	rac GTPase-activating protein 1 isoform a
NM_001320002.2	NP_001306931.1	rac GTPase-activating protein 1 isoform a
NM_001320003.2	NP_001306932.1	rac GTPase-activating protein 1 isoform a
NM_001320004.2	NP_001306933.1	rac GTPase-activating protein 1 isoform a
NM_001320005.2	NP_001306934.1	rac GTPase-activating protein 1 isoform b
NM_001320006.2	NP_001306935.1	rac GTPase-activating protein 1 isoform c
NM_001320007.2	NP_001306936.1	rac GTPase-activating protein 1 isoform d
NM_013277.5	NP_037409.2	rac GTPase-activating protein 1 isoform a

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables GTPase activator activity	IDA IDA: Inferred from direct assay	10979956	GOA
enables GTPase activator activity	IMP IMP: Inferred from mutant phenotype	15642749	GOA
enables alpha-tubulin binding	IDA IDA: Inferred from direct assay	11085985	GOA
enables beta-tubulin binding	IDA IDA: Inferred from direct assay	11085985	GOA
enables gamma-tubulin binding	IDA IDA: Inferred from direct assay	11085985	GOA
enables microtubule binding	IDA IDA: Inferred from direct assay	11782313	GOA
enables phosphatidylinositol-3,4,5-trisphosphate binding	IDA IDA: Inferred from direct assay	23235882	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	11278976	GOA
enables protein kinase binding	IPI IPI: Inferred from physical interaction	19468302	GOA
enables protein-macromolecule adaptor activity	IDA IDA: Inferred from direct assay	11782313	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in actomyosin contractile ring assembly	IMP IMP: Inferred from mutant phenotype	16129829	GOA
involved in erythrocyte differentiation	IMP IMP: Inferred from mutant phenotype	34818416	GOA
involved in mitotic cytokinesis	IDA IDA: Inferred from direct assay	11085985	GOA
involved in mitotic cytokinesis	IMP IMP: Inferred from mutant phenotype	16236794	GOA
involved in mitotic spindle midzone assembly	IDA IDA: Inferred from direct assay	16103226	GOA
involved in positive regulation of cytokinesis	IDA IDA: Inferred from direct assay	16103226	GOA
involved in positive regulation of cytokinesis	IMP IMP: Inferred from mutant phenotype	11782313	GOA
involved in regulation of attachment of spindle microtubules to kinetochore	IMP IMP: Inferred from mutant phenotype	15642749	GOA
involved in spermatogenesis	IEP IEP: Inferred from expression pattern	12590651	GOA
involved in sulfate transmembrane transport	IDA IDA: Inferred from direct assay	11278976	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of centralspindlin complex	IDA IDA: Inferred from direct assay	11782313	GOA
located in cleavage furrow	IDA IDA: Inferred from direct assay	16103226	GOA
located in cytoplasmic side of plasma membrane	IDA IDA: Inferred from direct assay	23235882	GOA
located in midbody	IDA IDA: Inferred from direct assay	11782313	GOA
located in mitotic spindle	IDA IDA: Inferred from direct assay	16103226	GOA
located in mitotic spindle	IMP IMP: Inferred from mutant phenotype	15642749	GOA
located in nucleus	IDA IDA: Inferred from direct assay	11085985	GOA
located in spindle	IDA IDA: Inferred from direct assay	18445686	GOA
located in spindle midzone	IDA IDA: Inferred from direct assay	19468302	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

RACGAP1 Protein Structure

C1_1

RhoGAP

0
100
200
300
400
500
600
632 a.a.

Protein Preferred Names	Protein Names
rac GTPase-activating protein 1	male germ cell RacGap

RACGAP1 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	RACGAP1	Q9H0H5	ECT2	Homo sapiens	Q9H8V3	Anti Bait CoIP	18201571
Intra	RACGAP1	Q9H0H5	ECT2	Homo sapiens	Q9H8V3	ITC	25068414
Intra	RACGAP1	Q9H0H5	ECT2	Homo sapiens	Q9H8V3	Anti Bait CoIP	19468302
Intra	RACGAP1	Q9H0H5	SLC26A8	Homo sapiens	Q96RN1	Pull Down	11278976
Intra	RACGAP1	Q9H0H5	KIF23	Homo sapiens	Q02241	Anti Tag CoIP	35271311
Intra	RACGAP1	Q9H0H5	KIF23	Homo sapiens	Q02241	IF	11782313
Intra	RACGAP1	Q9H0H5	KIF23	Homo sapiens	Q02241	Anti Tag CoIP	26496610
Intra	RACGAP1	Q9H0H5	KIF23	Homo sapiens	Q02241	Anti Tag CoIP	33961781
Intra	RACGAP1	Q9H0H5	KIF23	Homo sapiens	Q02241	Anti Tag CoIP	32203420
Intra	RACGAP1	Q9H0H5	KIF23	Homo sapiens	Q02241	Anti Bait CoIP	11782313
Intra	RACGAP1	Q9H0H5	MORF4L1	Homo sapiens	Q9UBU8	Y2H Prey Pooling	25416956
Intra	RACGAP1	Q9H0H5	PLK1	Homo sapiens	P53350	Anti Bait CoIP	19468302
Intra	RACGAP1	Q9H0H5	CTNNA1	Homo sapiens	P35221	Anti Bait CoIP	22750944
Intra	RACGAP1	Q9H0H5	TRAF3IP3	Homo sapiens	Q9Y228	Validated Y2H	32296183
Intra	RACGAP1	Q9H0H5	RAB11FIP3	Homo sapiens	O75154	Competition Binding	18511905

Cross: Cross-species interaction Intra: Intraspecies interaction

RACGAP1 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P810294	RACGAP1 Antibody (YA9637)	WB	Human, Mouse, Rat
HY-P83136	RACGAP1 Antibody (YA2881)	WB, IHC-P, ICC/IF	Human

Related Diseases

Diseases	Alias
Anemia, Congenital Dyserythropoietic, Type Iiib, Autosomal Recessive	CDAN3B Cda, Type Iiib Anemia, Congenital Dyserythropoietic, 3b, Autosomal Recessive
Cataract 36	CTRCT36 Catc4 Autosomal Recessive Congenital Cataract 4 Cataract, Autosomal Recessive Congenital 4 Cataract, Type 36
Anemia, Congenital Dyserythropoietic, Type Iiia	Congenital Dyserythropoietic Anemia, Type Iii Cdan3 Congenital Dyserythropoietic Anemia Type Iii Cda Iii Congenital Dyserythropoietic Anemia Type 3 CDAN3A Dyserythropoietic Anemia, Congenital, Type Iiia Cda, Type Iiia Anemia, Congenital Dyserythropoietic, Type Iii Anemia With Multinucleated Erythroblasts Cda Type 3 Cda Type Iii Dyserythropoietic Anemia, Congenital Type 3 Cda, Type Iii Erythroreticulosis, Hereditary Benign Anaemia With Multinucleated Erythroblasts Congenital Dyserythropoietic Anaemia Type 3 Congenital Dyserythropoietic Anaemia Type Iii Hereditary Benign Erythroreticulosis Anemia, Congenital Dyserythropoietic, 3a Anemia With Multinucleated Erythroblasts Erythroreticulosis, Hereditary Benign
Hepatocellular Carcinoma	Liver Cancer Primary Liver Cancer HCC Hepatoma Malignant Neoplasm Of Liver Liver Neoplasms Cancer, Hepatocellular Liver Cell Carcinoma Lcc Hepatoblastoma, Somatic Hepatic Cancer Primary Malignant Neoplasm Of Liver Rare Tumor Of Liver And Intrahepatic Biliary Tract Hepatocellular Carcinoma, Somatic Hepatocellular Carcinoma, Childhood Type, Somatic Hepatocellular Cancer, Somatic Ca Liver - Primary Hepatic Neoplasm Malignant Hepato-Biliary Neoplasm Malignant Neoplasm Of Liver, Not Specified As Primary Or Secondary Malignant Neoplasm Of Liver, Primary Malignant Tumor Of Liver Neoplasm Of Liver Non-Resectable Primary Hepatic Malignant Neoplasm Resectable Malignant Neoplasm Of Liver Resectable Malignant Neoplasm Of The Liver Primary Liver Carcinoma Primary Malignant Liver Neoplasm Primary Cancer Of Liver Primary Tumor Of The Liver Rare Tumor Of Liver And Ibt Hepatocellular Cancer Neoplasm Of The Liver Carcinoma, Hepatocellular Hepatomas Liver Neoplasm Liver Carcinoma Liver And Intrahepatic Biliary Tract Carcinoma Malignant Hepatobiliary Neoplasm Adult Primary Hepatocellular Carcinoma Hepatoblastoma Carcinoma Of Liver Malignant Liver Tumour Malignant Hepatic Tumour
Uterine Body Mixed Cancer	Malignant Mixed Tumor Of Corpus Uteri Malignant Mixed Tumor, Not Otherwise Specified
Uterine Carcinosarcoma	Carcinosarcoma Of The Corpus Uteri Mixed Mullerian Sarcoma Of Uterus Malignant Mixed Müllerian Tumor Of The Corpus Uteri Malignant Mixed Müllerian Tumor Of Corpus Uteri Mixed Müllerian Cancer Of Corpus Uteri Malignant Mixed Mullerian Tumor Of The Corpus Uteri Mixed Mullerian Cancer Of Corpus Uteri Uterine Corpus Carcinosarcoma

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (3)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS11594	RACGAP1 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS19840	Racgap1 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS26337	Racgap1 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Bos taurus	RACGAP1	VGNC	VGNC:33675
Macaca mulatta	RACGAP1	VGNC	VGNC:100098
Felis catus	RACGAP1	VGNC	VGNC:69210
Rattus norvegicus	RACGAP1	RGD	RGD:1305912
Canis familiaris	RACGAP1	VGNC	VGNC:45308
Mus musculus	RACGAP1	MGD	MGI:1349423
Others	RACGAP1	NCBI