IRF8 Antibody (YA9636)
(Synonyms: ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP)IRF8 Antibody (YA9636) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRF8.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IP, FC
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:2,000 | 1:100 | 1:1,000 | 1-2μg/sample |
Product Details
IRF8 Antibody (YA9636) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRF8.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 48 kDa
Recombinant protein within human IRF8 aa 81-426.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
IRF8 is a Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Can both act as a transcriptional activator or repressor (By similarity). Plays a negative regulatory role in cells of the immune system (By similarity). Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity). Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (By similarity). Positively regulates macroautophagy in dendritic cells. Acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1 (By similarity)[1][2].
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Subcellular Localization
Nucleus; Cytoplasm
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Expression
Tissue_Specificity: Predominantly expressed in lymphoid tissues.
Induction: By IFNG/IFN-gamma. Negatively regulated by microRNA-155 (miR155). -
Isoforms & Post-Translational Modification
IRF8 has an amino acid length of 426, molecular weight is 48356 Da.
Ubiquitinated. Ubiquitination leads to its degradation by the proteasome -
Subunit
Interacts (via C-terminus) with TRIM21 (via C-terminus).
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SwissProt ID
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Synonyms
ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP
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Research Field
Epigenetics and Nuclear Signaling
Documentation
References
[1]. Salem S, et al. Functional characterization of the human dendritic cell immunodeficiency associated with the IRF8(K108E) mutation. Blood. 2014 Sep 18;124(12):1894-904. [Content Brief]
[2]. Agod Z, et al. Signaling Lymphocyte Activation Molecule Family 5 Enhances Autophagy and Fine-Tunes Cytokine Response in Monocyte-Derived Dendritic Cells via Stabilization of Interferon Regulatory Factor 8. Front Immunol. 2018;9:62. [Content Brief]
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