BRD9 Antibody (YA9635)
(Synonyms: Bromodomain containing 9 antibody; Bromodomain containing protein 9 antibody; DKFZp434D0711 antibody; DKFZp686L0539 antibody; FLJ13441 antibody; LAVS3040 antibody; PRO9856 antibody; Rhabdomyosarcoma antigen MU RMS 40.8 antibody; Sarcoma antigen NY SAR 29 antibody; UNQ3040 antibody)BRD9 Antibody (YA9635) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRD9.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP
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Reactivity :
Human, Mouse, Rat, Monkey
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IFl
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:5,000 | 1:200 | 1:50 | 1-2μg/sample |
Product Details
BRD9 Antibody (YA9635) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRD9.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat, Monkey
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Observed Molecular WeightObserved band size: 75 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 67 kDa
Recombinant protein within human BRD9 aa 201-597.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
BRD9 plays a role in chromatin remodeling and regulation of transcription. Acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated. Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Also orchestrates the RAD51-RAD54 complex formation and thereby plays a role in homologous recombination (HR)[1][2][3][4].
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Subcellular Localization
Nucleus
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Isoforms & Post-Translational Modification
BRD9 has 6 isoforms, Q9H8M2-5: amino acid length is 597, molecular weight is 67000 Da (predicted); Q9H8M2-2: amino acid length is 206, molecular weight is 22534 Da (predicted); Q9H8M2-3: amino acid length is 481, molecular weight is 53249 Da (predicted); Q9H8M2-4: amino acid length is 233, molecular weight is 26247 Da (predicted); Q9H8M2-1: amino acid length is 501, molecular weight is 55665 Da (predicted); Q9H8M2-6: amino acid length is 544, molecular weight is 60763 Da (predicted).
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Subunit
Binds acetylated histones H3 and H4.
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SwissProt ID
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Synonyms
Bromodomain containing 9 antibody; Bromodomain containing protein 9 antibody; DKFZp434D0711 antibody; DKFZp686L0539 antibody; FLJ13441 antibody; LAVS3040 antibody; PRO9856 antibody; Rhabdomyosarcoma antigen MU RMS 40.8 antibody; Sarcoma antigen NY SAR 29 antibody; UNQ3040 antibody
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Research Field
Signal Transduction
Documentation
References
[1]. Filippakopoulos P, et al. Histone recognition and large-scale structural analysis of the human bromodomain family. Cell. 2012 Mar 30;149(1):214-31. [Content Brief]
[2]. Flynn EM, et al. A Subset of Human Bromodomains Recognizes Butyryllysine and Crotonyllysine Histone Peptide Modifications. Structure. 2015 Oct 6;23(10):1801-1814. [Content Brief]
[3]. Alpsoy A, et al. Glioma tumor suppressor candidate region gene 1 (GLTSCR1) and its paralog GLTSCR1-like form SWI/SNF chromatin remodeling subcomplexes. J Biol Chem. 2018 Mar 16;293(11):3892-3903. [Content Brief]
[4]. Zhou Q, et al. The bromodomain containing protein BRD-9 orchestrates RAD51-RAD54 complex formation and regulates homologous recombination-mediated repair. Nat Commun. 2020 May 26;11(1):2639. [Content Brief]
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