CTNNA1 Antibody (YA7681)

Cat. No.: HY-P87996: Data Sheet User Guide for Antibodies
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CTNNA1 Antibody (YA7681) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CTNNA1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CTNNA1 Antibody (YA7681) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CTNNA1.

Host

Rabbit

Clonality

Recombinant

Molecular Weight

Predicted band size: 100 kDa;

Observed band size: 100 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P35221

Immunogen

A synthesized peptide derived from human alpha Catenin

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200
FC FC: Flow Cytometry	1:50-100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
IP IP: Immunoprecipitation	1:20-50

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Solution

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). May play a crucial role in cell differentiation

Subcellular Localization：Cytoplasm, cytoskeleton,Cell junction, adherens junction,Cell membrane,Cell junction,Cytoplasm,Nucleus,Cell membrane

Expression：
Tissue_Specificity: Ubiquitously expressed in normal tissues|Abundantly expressed in brain and cerebellum, also expressed in the placenta, liver, lung, colon, heart, pancreas, stomach and thymus

Isoforms & Post-Translational Modification：P35221 has three isomers: P35221-1: 100071 Da (predicted); P35221-2: 102635 Da (predicted); P35221-3: 59550 Da (predicted).
Sumoylated丨Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion

Subunit：Monomer and homodimer; the monomer preferentially binds to CTNNB1 and the homodimer to actin (By similarity)

Synonyms

Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13, CTNNA1

Documentation

User Guide for Antibodies (1077 KB)