Transmembrane Protein 85 Antibody (YA7684)
(Synonyms: TMEM85, HSPC184, PIG17, EMC4, ER membrane protein complex subunit 4, Cell proliferation-inducing gene 17 protein, Transmembrane protein 85)
Based on 1 Customer Validation
Transmembrane Protein 85 Antibody (YA7684) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transmembrane Protein 85.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, IP, IF-Tissue
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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IP
IP: Immunoprecipitation
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:100-500 | 1:20-50 | 1:100-500 |
Product Details
Transmembrane Protein 85 Antibody (YA7684) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transmembrane Protein 85.
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Host Rabbit
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Clonality Recombinant
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Species ReactivityHuman, Mouse, Rat Predicted Reactivity: Mouse,Rat
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
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Observed Molecular WeightObserved band size: 17 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 20 kDa
A synthesized peptide derived from human EMC4: 22-65.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Transmembrane Protein 85 is a Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable)[1][2][3][4][5].
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Subcellular Localization
Endoplasmic reticulum membrane
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Expression
Tissue_Specificity: Isoform 1 is expressed in brain and heart. Isoform 2 is expressed in heart -
Isoforms & Post-Translational Modification
Q5J8M3 has three isomers: Q5J8M3-1: 20087 Da (predicted); Q5J8M3-2: 16745 Da (predicted); Q5J8M3-3: 15088 Da (predicted).
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Subunit
Component of the ER membrane protein complex (EMC)
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SwissProt ID
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Synonyms
TMEM85, HSPC184, PIG17, EMC4, ER membrane protein complex subunit 4, Cell proliferation-inducing gene 17 protein, Transmembrane protein 85
Documentation
References
[1]. Guna A, et al. The ER membrane protein complex is a transmembrane domain insertase. Science. 2018 Jan 26;359(6374):470-473. [Content Brief]
[2]. Shurtleff MJ, et al. The ER membrane protein complex interacts cotranslationally to enable biogenesis of multipass membrane proteins. Elife. 2018 May 29;7:. [Content Brief]
[3]. Chitwood PJ, et al. EMC Is Required to Initiate Accurate Membrane Protein Topogenesis. Cell. 2018 Nov 29;175(6):1507-1519.e16. [Content Brief]
[4]. Pleiner T, et al. Structural basis for membrane insertion by the human ER membrane protein complex. Science. 2020 Jul 24;369(6502):433-436. [Content Brief]
[5]. O'Donnell JP, et al. The architecture of EMC reveals a path for membrane protein insertion. Elife. 2020 May 27;9:. [Content Brief]
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