Transmembrane Protein 85 Antibody (YA7684)

Art. -Nr.: HY-P87999: Data Sheet User Guide for Antibodies
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Transmembrane Protein 85 Antibody (YA7684) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transmembrane Protein 85.

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

Transmembrane Protein 85 Antibody (YA7684) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transmembrane Protein 85.

Host

Rabbit

Clonality

Recombinant

Molekulargewicht

Predicted band size: 20 kDa;

Observed band size: 18 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human Predicted Reactivity: Mouse,Rat

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

Q5J8M3

Immunogen

A synthesized peptide derived from human EMC4

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-200
IP IP: Immunoprecipitation	1:20-50

Sensitivity	Endogenous	Reinheit	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Solution

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:29242231, PubMed:29809151, PubMed:30415835, PubMed:32439656, PubMed:32459176). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:29242231, PubMed:29809151, PubMed:30415835). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:29809151, PubMed:30415835). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29242231, PubMed:29809151). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable)

Subcellular Localization：Endoplasmic reticulum membrane

Expression：
Tissue_Specificity: Isoform 1 is expressed in brain and heart. Isoform 2 is expressed in heart

Isoforms & Post-Translational Modification：Q5J8M3 has three isomers: Q5J8M3-1: 20087 Da (predicted); Q5J8M3-2: 16745 Da (predicted); Q5J8M3-3: 15088 Da (predicted).

Subunit：Component of the ER membrane protein complex (EMC)

Synonyms

TMEM85, HSPC184, PIG17, EMC4, ER membrane protein complex subunit 4, Cell proliferation-inducing gene 17 protein, Transmembrane protein 85

Dokumentation

User Guide for Antibodies (1077 KB)