Delivery of miRNA-29b Using R9-LK15, a Novel Cell-Penetrating Peptide, Promotes Osteogenic Differentiation of Bone Mesenchymal Stem Cells

  • Biomed Res Int. 2019 Apr 11;2019:3032158. doi: 10.1155/2019/3032158.
Qiuling Liu  1 ,  Zhen Lin  1 ,  Yi Liu  2 ,  Jiang Du  1 ,  Hongsheng Lin  1 ,  Jing Wang  1
Affiliations
  • 1. Department of Orthopedics, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong Province, China.
  • 2. Affiliated Stomatology Hospital of Guangzhou Medical University, School of Stomatology, Guangzhou Medical University, Guangzhou, Guangdong Province, China.
PMID: 31111046 DOI: 10.1155/2019/3032158
Abstract

Delivery of osteogenesis-promoting MicroRNAs (miRNAs) is a promising approach to enhance bone regeneration. In this study, we generated nanocomplexes comprising the novel cell-penetrating peptide R9-LK15 and miR-29b and investigated their effects on Osteogenic Differentiation of bone mesenchymal stem cells (BMSCs). R9-LK15/miR-29b nanocomplexes were prepared and characterized. The transfection efficiency, cell viability, and Osteogenic Differentiation were investigated. The results showed that R9-LK15 maintained the stability of miR-29b in serum for up to 24 h. Moreover, R9-LK15 efficiently delivered miR-29b into BMSCs; the transfection efficiency was ~10-fold higher than that achieved using Lipofectamine 2000. The Cell Counting Kit-8 assay showed that R9-LK15 and R9-LK15/miR-29b nanocomplexes had negligible cytotoxic effects on BMSCs. Delivery of R9-LK15/miR-29b nanocomplexes promoted Osteogenic Differentiation of BMSCs and extracellular matrix mineralization by upregulating Alkaline Phosphatase expression and downregulating histone deacetylase-4 expression. In general, we developed a novel miRNA delivery system that has a high transfection efficiency and promotes Osteogenic Differentiation.

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