PKM2 knockdown influences SREBP activation and lipid synthesis in bovine mammary-gland epithelial MAC-T cells

  • Biotechnol Lett. 2018 Apr;40(4):641-648. doi: 10.1007/s10529-018-2518-3.
Bing-Qian Su  1 ,  Ying-Qian Han  1 ,  Shuang-Shuang Fan  1 ,  Sheng-Li Ming  1 ,  Bo Wan  1 ,  Wei-Fei Lu  1 ,  Bei-Bei Chu  1 ,  Guo-Yu Yang  1 ,  Jiang Wang  2
Affiliations
  • 1. College of Animal Sciences and Veterinary Medicine, Henan Agricultural University, No. 95, Wenhua Road, Zhengzhou, 450002, Henan, People's Republic of China.
  • 2. College of Animal Sciences and Veterinary Medicine, Henan Agricultural University, No. 95, Wenhua Road, Zhengzhou, 450002, Henan, People's Republic of China. [email protected].
PMID: 29383471 DOI: 10.1007/s10529-018-2518-3
Abstract

Objective: The purpose of the article is to evaluate the changes in lipid metabolism in bovine mammary-gland epithelial MAC-T cells after PKM2 knockdown.

Results: MAC-T cells stably expressing low levels of PKM2 were established with lentivirus-mediated small hairpin RNA. Although the knockdown of PKM2 had no effect on MAC-T cell growth, the reduced expression of PKM2 attenuated the mRNA and protein expression of key Enzymes involved in sterol synthesis through the SREBP pathway.

Conclusions: The downregulation of PKM2 significantly influenced lipid synthesis in bovine mammary-gland epithelial MAC-T cells. These findings extend our understanding of the crosstalk between glycolysis and lipid metabolism in bovine mammary-gland epithelial cells.

Keywords
Bovine mammary-gland epithelial cells; Lipid synthesis; PKM2; SREBP.
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