Regulation of lipoxygenase-1 and Dectin-1 on interleukin-10 in mouse Aspergillus fumigatus keratitis

  • Int J Ophthalmol. 2018 Jun 18;11(6):905-909. doi: 10.18240/ijo.2018.06.02.
Cheng-Ye Che  1 ,  Ke-Lan Yuan  1 ,  Gui-Qiu Zhao  1 ,  Cui Li  1 ,  Jing Lin  1 ,  Guo-Qiang Zhu  1 ,  Min Liu  1
Affiliations
  • 1. Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China.
PMID: 29977799 DOI: 10.18240/ijo.2018.06.02
Abstract

Aim: To investigate the regulation of Lipoxygenase (LOX)-1 and Dectin-1 on interleukin-10 (IL-10) production in mice with Aspergillus fumigatus (A. fumigatus) keratitis.

Methods: The corneas of C57BL/6 mice were pretreated with LOX-1 Inhibitor Poly(I) or Dectin-1 siRNA separately before the Infection of A. fumigatus. Polymerase chain reaction (PCR) and Western blot were used to detect the expression of IL-10.

Results: The mRNA and protein expressions of IL-10 were significantly increased in mice with A. fumigatus keratitis. Compared with the group pretreated with sterile water before Infection, Poly(I) pretreatment suppressed IL-10 expression significantly. Compared with the group pretreated with scrambled siRNA before Infection, Dectin-1 siRNA pretreatment significantly reduced IL-10 expression in response to A. fumigatus Infection.

Conclusion: LOX-1 and Dectin-1 regulate IL-10 production in mouse A. fumigatus keratitis.

Keywords
Aspergillus fumigatus; Dectin-1; interleukin-10; keratitis; lipoxygenase-1.
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