Trypsin (MS grade)
Based on 1 publication(s) in Google Scholar
Trypsin MS grade is a serine protease enzyme, and hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin MS grade activates PAR2 and PAR4. Trypsin MS grade induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin MS grade also promotes cell proliferation and differentiation. Trypsin MS grade can be used in the research of wound healing and neurogenic inflammation.
Para uso exclusivo en investigación. No vendemos a pacientes.
- No. CAS: 9002-07-7
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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HY-P1645
PapainPapain -
HY-B2118
PancreatinPancreatic Digestive Enzymes -
HY-129047C
TPCK-treated TrypsinBiochemical Assay Reagent -
HY-129047
TrypsinSerine Protease -
HY-E70005A
Collagenase, Type IZinc peptidase, Matrix metalloproteinases (MMPs) -
HY-129047E
Trypsin, porcine pancreas (Cell culture grade)Serine Protease -
HY-129047B
Recombinant Trypsin SolutionTrypsin Solution -
HY-P3028
FicainEnzyme Extract -
HY-P1635
PepsinGastric Protease -
HY-E70198
Pepsin (MS Grade)Gastric Protease -
HY-125859
Peroxidase, HorseradishPeroxidase
Publications Citing Use of MedChemExpress (MCE) Trypsin (MS grade)
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Actividad biológica
Descripciòn
IC50 & Target
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PAR2 |
PAR4 |
In Vitro
Trypsin MS grade (5 μg/mL, 24 or 48 h) promotes porcine deltacoronavirus (PDCoV) replication in LLC-PK cells[2].
Trypsin MS grade (10 and 50 ng/mL, 12 h) enhances PDCoV cell-to-cell spread in LLC-PK cells by promoting membrane fusion in LLC-PK cells[2].
Trypsin MS grade (0.05%, 3 h) promotes C6 glioma cell proliferation in serum-free and growth factor-free medium[3].
Trypsin MS grade (20 -150 ng/mL, 5 days) potentiates PBMC differentiation[4].
This product can be used for protein sequence analysis, such as protein mass spectrometry, sequencing, and peptide map analysis.
Protocol
This product is recommended to be dissolved or diluted with 1 mM HCl. The recommended enzymatic digestion ratio is recombinant trypsin: target protein = 1:20-1:100, and the optimal pH is 7.0-8.0.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- Research Protocol for Infectious Diseases
- BrdU Incorporation Assay
- Cell migration
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Research Protocol for Inflammation-related Diseases
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- MTT Cell Proliferation Assay
- Cell-Exclusion Zone Migration Assay
EC Number
3.4.21.4
Enzyme Activity
≥6200 USP U/mg soild
Unit Definition
One USP unit is defined as the amount of enzyme that at 25°C and pH 7.6, in reaction system 3.2 mL (1 cm light path), hydrolysis of BAEE per minute increased the absorption value at 253 nm by 0.003.
Chemical Information
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No. CAS 9002-07-7
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Appearance Solid
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Color White to off-white
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SMILES
[Trypsin (MS grade)]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
Pureza y Documentación
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Ficha de datos (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[2]. Yue-Lin Yang,et al. Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner. Emerg Microbes Infect. 2020 Feb 24;9(1):457-468. [Content Brief]
[3]. H Amano, et al. Trypsin promotes C6 glioma cell proliferation in serum- and growth factor-free medium. Neurosci Res. 1996 Jul;25(3):203-8. [Content Brief]
[5]. R Costa, et al. Evidence for the role of neurogenic inflammation components in trypsin-elicited scratching behaviour in mice. Br J Pharmacol. 2008 Jul;154(5):1094-103. [Content Brief]
[6]. F Schmidlin, et al. Protease-activated receptors: how proteases signal to cells. Curr Opin Pharmacol. 2001 Dec;1(6):575-82. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Trypsin (MS grade) Related Classifications
HY-129047A Related Classifications
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