Recombinant Trypsin Solution

Instructions
Example: Culturing stem cells.
1. Remove the Recombinant Trypsin Solution and allow it to equilibrate to room temperature.
2. When stem cell cultures reach 80%-90% confluence, proceed with passaging or harvesting.
3. Aspirate the culture medium, rinse the cells with calcium- and magnesium-free PBS, and discard the PBS.
4. Add an appropriate volume of Recombinant Trypsin Solution, ensuring it covers the entire growth surface. Incubate at 37°C (or room temperature) for 3-5 minutes. Monitor under a microscope until the cells retract, become rounded, and detach. Add a volume of culture medium (twice the volume of the trypsin solution used) to stop the reaction. Pipette the suspension up and down to disperse the cells into a single-cell suspension, then perform a cell count.
Note: If most cells have retracted and become rounded but have not yet detached, gently tap the side of the culture vessel to dislodge them. If the cells still do not detach, extend the incubation time.
5. Centrifuge at 300 × g for 5 minutes, discard the supernatant, and collect the cells for subsequent experiments.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.