1. GPCR/G Protein
  2. Protease Activated Receptor (PAR)
  3. Trypsin

Trypsin 

Cat. No.: HY-129047
Handling Instructions

Trypsin is an enzyme that hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin activates PAR2 and PAR4. Trypsin induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin also promotes cell proliferation and differentiation. Trypsin can be used in the research of wound healing and neurogenic inflammation.

For research use only. We do not sell to patients.

Trypsin Chemical Structure

Trypsin Chemical Structure

CAS No. : 9002-07-7

Size Stock
100 mg   Get quote  
250 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Trypsin is an enzyme that hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin activates PAR2 and PAR4. Trypsin induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin also promotes cell proliferation and differentiation. Trypsin can be used in the research of wound healing and neurogenic inflammation[1][2][3][4][6].

IC50 & Target

PAR2, PAR4[6]

In Vitro

Trypsin (5 μg/mL, 24 or 48 h) promotes porcine deltacoronavirus (PDCoV) replication in LLC-PK cells[2].
Trypsin (10 and 50 ng/mL, 12 h) enhances PDCoV cell-to-cell spread in LLC-PK cells by promoting membrane fusion in LLC-PK cells[2].
Trypsin (0.05%, 3 h) promotes C6 glioma cell proliferation in serum-free and growth factor-free medium[3].
Trypsin (20 -150 ng/mL, 5 days) potentiates PBMC differentiation[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[2]

Cell Line: LLC-PK cells, ST cells
Concentration: 5 μg/mL
Incubation Time: 24 or 48 h
Result: Promoted PDCoV replication in LLC-PK cells but not ST cells.

Immunofluorescence[2]

Cell Line: LLC-PK cells, ST cells
Concentration: 10 and 50 ng/mL
Incubation Time: 12 h
Result: Significantly increased cell-to-cell fusion activity during PDCoV infection of LLC-PK cells.
In Vivo

Trypsin (100-500 μg per site in 50 μL saline, intradermal injection) induces scratching behaviour in mice[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Swiss mice[5]
Dosage: 100-500 μg per site, in saline (50 μL)
Administration: Intradermal injection
Result: Induced pruritus, and was inhibited by trypsin inhibitor.
CAS No.
SMILES

[Trypsin]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Trypsin
Cat. No.:
HY-129047
Quantity:
MCE Japan Authorized Agent: