Thermolysin, Bacillus thermoproteolyticus rokko
Based on 3 publication(s) in Google Scholar
Thermolysin, Bacillus thermoproteolyticus rokko (EC 3.4.24.27) (TML) is a thermostable neutral metalloproteinase enzyme secreted by the Gram-positive bacteria Bacillus thermoproteolyticus. Thermolysin catalyzes the hydrolysis of peptide bonds containing hydrophobic residues.
Optimal pH: 8.0. Considerably stable from pH 5 to 9.5.
Optimal temperature : 70 °C
For research use only. We do not sell to patients.
- CAS No.: 9073-78-3
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Thermolysin, Bacillus thermoproteolyticus rokko
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Biological Activity
Optimal pH: 8.0. Considerably stable from pH 5 to 9.5.
Optimal temperature : 70 °C
Thermolysin (100 μg in 1 mL, 64°C for 4 h, in isolated starch granules) digestion results in the selective removal of a group of low-molecular-mass proteins ranging between 10 and 27 kD[2].
Thermolysin can be used for the hydrolysis of bovine liver sarcoplasmic proteins[3].
Product Information
Molecular weight: 34.6 kDa (calculated)
Optimum pH: 8 0, stable in the pH range of 5.0-9 5
Optimum temperature: 70°C
Isoelectric point: 4.45
Instructions
Soluble in acetate buffer, pH 7.5 (0.2 mg/mL), forming a clear to slightly turbid colorless solution. Alternatively, use pure water or buffer according to the specific experimental reference.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Thermolysin is not acutely toxic with an oral LD50 of more than 18000 mg/kg in rats and more than 24000 mg/kg in mice (Acute toxicity studies)[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Spontaneously hypertensive rats (SHR) rats[4]
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Dosage:50 mg/kg
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Administration:Oral administration (p.o.), once
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Result:Lowered systolic blood pressure in SHR rats, with maximal reduction by 22 mm in systolic blood pressure being observed 6 h after administration.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
3.4.24.27
30-350 U/mg protein
One unit is defined as the amount of hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) 7.5g of tyrosine per min at pH 7.5 at 37°C.(Folin Jockett reagent)
Chemical Information
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CAS No. 9073-78-3
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Appearance Solid
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Color White to light yellow
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SMILES
[Thermolysin]
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Synonyms
EC 3.4.24.27; TML
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (3)
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Journal Impact Factor
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Most Recent
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Sci Rep
Polysaccharide from Prinsepia utilis Royle maintains the skin barrier by mediating differentiation, lipid metabolism and tight junction of keratinocyte. [Abstract]2025 Jul 1;15(1):20470. PMID: 40595762 -
J Mol Neurosci
2024 Jun 21;74(3):60. PMID: 38904846 -
bioRxiv
2025 Feb 20:2025.02.18.638921. PMID: 40027750
Solvent & Solubility
H2O : ≥ 1 mg/mL
* "≥" means soluble, but saturation unknown.
Purity & Documentation
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Data Sheet (288 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
[1]. Bertusvan den Burg, et al. Chapter 111 - Thermolysin and Related Bacillus Metallopeptidases.
[2]. Mu-Forster C, et al. Surface localization of zein storage proteins in starch granules from maize endosperm. Proteolytic removal by thermolysin and in vitro cross-linking of granule-associated polypeptides. Plant Physiol. 1998 Apr;116(4):1563-71. [Content Brief]
[3]. Di Bernardini R, et al. Isolation, purification and characterization of antioxidant peptidic fractions from a bovine liver sarcoplasmic protein thermolysin hydrolyzate. Peptides. 2011 Feb;32(2):388-400. [Content Brief]
[4]. Fujita H, et al: a prodrug-type ACE-inhibitory peptide derived from fish protein. Immunopharmacology. 1999 Oct 15;44(1-2):123-7. [Content Brief]
[5]. Ke Q, et al. Safety evaluation of a thermolysin enzyme produced from Geobacillus stearothermophilus. Food Chem Toxicol. 2013 Sep;59:541-8. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Thermolysin, Bacillus thermoproteolyticus rokko Related Classifications
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