TLCK-treated Chymotrypsin
Based on 3 publication(s) in Google Scholar
TLCK-treated Chymotrypsin is a serine protease. Chymotrypsin cleaves protein chains at the carboxyl side of aromatic amino acids. TLCK treated to inactivate residual tryspin activity.
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- No. CAS: 9004-07-3
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Publications Citing Use of MedChemExpress (MCE) TLCK-treated Chymotrypsin
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Actividad biológica
Descripciòn
In Vitro
Recommended to reconstitute in 1mM HCl at 10 mg/mL and add 2 mM calcium chloride as stabilizer. Resuspended Chymotrypsin can be stored for up to one week at 4°C Preparation of Protein 1. Solubilization/Denaturation: Dissolve protein in 100 mM Tris-HCl, 10 mM CaCl2 (pH 8.0). Proteins that are difficult to dissolve or require denaturation for efficient digestion can be solubilized in a minimum volume in a denaturant such as 6–8 M urea or 6 M guanidine HCl at room temperature to 37°C for up to one hour. For some proteins, it may be beneficial to heat the sample to 60°C over this time period (95°C for 15–20 minutes for extreme cases). 2. Disulphide Reduction: To the dissolved protein add DTT (or β-mercaptoethanol) to a final concentration of 5mM; heat this sample at 50–60°C for 20 minutes. 3. Alkylation: Allow the reduced protein mixture to cool to room temperature, and add iodoacetamide to a final concentration of 15 mM. Incubate in the dark for 15 minutes at room temperature. 4. Finally adjust the reaction volume with 100 mM Tris-HCl, 10 mM CaCl2 (pH 8.0) such that the urea or guanidine concentration is 1 M or less. Enzyme Reconstitution Dissolve Chymotrypsin in 1mM HCl. We recommend a final concentration 0.5–1 μg/μl. Digestion Add Chymotrypsin to a final protease:protein ratio of 1:200 to 1:20 (w/w), and incubate sample for 2–18 hours at 25°C. The reaction may be stopped, if desired, by adding 0.5% trifluoroacetic acid.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Specific Activity
≥45 U/mg protein
Unit Definition
One unit is defined as the amount of enzyme that can hydrolyzes 1 μmol of benzoyl-L-tyrosine ethyl ester per minute at 25°C, pH 7.8.
Chemical Information
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No. CAS 9004-07-3
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Appearance Solid
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Color White to off-white
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SMILES
[TLCK-treated Chymotrypsin]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (3)
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Journal Impact Factor
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Most Recent
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Nat Protoc
Design, performance, processing, and validation of a pooled CRISPR perturbation screen for bacterial toxins. [Abstract]2025 May;20(5):1158-1195. PMID: 39487259
TLCK-treated Chymotrypsin purchased from MedChemExpress. Usage Cited in: Nat Protoc. 2025 May;20(5):1158-1195. [Abstract]
Vibrio cholerae cytolysin (VCC) was produced as an inactive pro-toxin (80 kDa), and the mature toxin (65 kDa) was generated through proteolysis by Chymotrypsin (incubated with VCC at 1000:1 molar ratio; room temperature; 2 h). A representative gel for in-house produced His-tagged pro-VCC and its proteolysis activation was shown. Both the toxin purity (the major band was toxin, with few degradation bands) and activation efficiency were satisfactory.
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J Med Chem
Peptidomimetic Analogues Act as Effective Inhibitors against SARS-CoV-2 by Blocking the Function of Cathepsin L. [Abstract]2024 Oct 10;67(19):17124-17143. PMID: 39292661 -
PLoS Pathog
Serine protease-driven entry and S2 ' cleavage flexibility of feline coronavirus during feline enterocyte infections. [Abstract]2026 Jan 8;22(1):e1013854. PMID: 41505483
Solvente y solubilidad
In Vitro:
H2O : ≥ 100 mg/mL
* "≥" means soluble, but saturation unknown.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Pureza y Documentación
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Ficha de datos (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
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- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
TLCK-treated Chymotrypsin Related Classifications
HY-108910B Related Classifications
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