2. Academic Validation
  3. In vitro and in vivo activity of chelerythrine against Candida albicans and underlying mechanisms

In vitro and in vivo activity of chelerythrine against Candida albicans and underlying mechanisms

  • Future Microbiol. 2019 Dec;14:1545-1557. doi: 10.2217/fmb-2019-0178.
Ying Gong 1 2,  Siwen Li 3,  Weixin Wang 4,  Yiman Li 5,  Wenli Ma 5 6,  Shujuan Sun 3
Affiliations

Affiliations

  • 1 Department of Pharmacy, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, 250012, PR China.
  • 2 Department of Pharmacy, WuXi People's Hospital Affiliated with Nanjing Medical University, Wuxi, 214023, PR China.
  • 3 Department of Pharmacy, Shandong Provincial Qianfoshan Hospital, The First Hospital Affiliated with Shandong First Medical University, Jinan, 250014, PR China.
  • 4 Department of Pharmacy, Taishan Hospital of Shandong Province, Taian, 271000, PR China.
  • 5 School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, PR China.
  • 6 Department of Pharmacy, Feicheng Mining Central Hospital, Feicheng 271608, PR China.
PMID: 31992072 DOI: 10.2217/fmb-2019-0178
Abstract

Aim: To evaluate whether chelerythrine (CHT) exhibited Antifungal activity against Candida albicans in vitro and in vivo and to explore the underlying mechanisms. Materials & methods: Broth microdilution assay and Galleria mellonella model were used to evaluate the Antifungal effect in vitro and in vivo, respectively. Mechanism studies were investigated by morphogenesis observation, Fluo-3/AM, DCFH-DA and rhodamine6G assay, respectively. Results: CHT exhibited Antifungal activity against C. albicans and preformed biofilms with minimum inhibitory concentrations ranged from 2 to 16 μg/ml. Besides, CHT protected G. mellonella larvae infected by C. albicans. Mechanisms studies revealed that CHT inhibited hyphal growth, increased intracellular calcium concentration, induced accumulation of Reactive Oxygen Species and inhibited drug transporter activity. Conclusion: CHT exhibited Antifungal activity against C. albicans.

Keywords

Candida albicans; Galleria mellonella model; antifungal activity; chelerythrine; potential mechanism.

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