Cat. No.: HY-N0683

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α-Vitamin E

(Synonyms: (+)-α-Tocopherol; D-α-Tocopherol)

22 Cited Publications

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Based on 22 publication(s) in Google Scholar

α-Vitamin E ((+)-α-Tocopherol), a naturally occurring vitamin E form, is a potent antioxidant.

For research use only. We do not sell to patients.

Purity: 99.67%
CAS No.: 59-02-9
Formula: C₂₉H₅₀O₂
Molecular Weight:430.71

Storage:

4°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

α-Vitamin E Chemical Structure

CAS No.: 59-02-9

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (22)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

α-Vitamin E ((+)-α-Tocopherol), a naturally occurring vitamin E form, is a potent antioxidant^[1]^[2].

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Erythrocyte	IC₅₀	3.658 μg/mL Compound: alpha-tocopherol	Antioxidant activity in rat erythrocytes assessed as inhibition of phenyl hydrazine-induced haemolysis after 1 hr Antioxidant activity in rat erythrocytes assessed as inhibition of phenyl hydrazine-induced haemolysis after 1 hr	[PMID: 23395966]
Fibroblast	EC₅₀	234 nM Compound: 2, Vitamin E	Cytoprotective activity against BSO-induced human FRDA primary patient fibroblasts compound pretreated 12 hrs prior to BSO-induction measured after 48 hrs by calcein-AM-based cell viability assay Cytoprotective activity against BSO-induced human FRDA primary patient fibroblasts compound pretreated 12 hrs prior to BSO-induction measured after 48 hrs by calcein-AM-based cell viability assay	[PMID: 22137789]
Fibroblast	GI₅₀	≥300 μM Compound: 2, Vitamin E	Growth inhibition of human FRDA primary patient fibroblasts after 48 hrs by calcein-AM-based cell viability assay Growth inhibition of human FRDA primary patient fibroblasts after 48 hrs by calcein-AM-based cell viability assay	[PMID: 22137789]
Fibroblast	EC₅₀	>500 nM Compound: alpha-TOH	Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay	[PMID: 33214825]
HepG2	IC₅₀	>300 μM Compound: alpha-TOH	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]
HL-60	IC₅₀	255 μM Compound: Vitamin E	Antioxidant activity against TPA-induced ROS production in human HL60 cells by 2',7'-dichlorodihydrofluorescein diacetate cellular-based assay Antioxidant activity against TPA-induced ROS production in human HL60 cells by 2',7'-dichlorodihydrofluorescein diacetate cellular-based assay	[PMID: 12762791]
J774	IC₅₀	>500 μM Compound: 1	Cytotoxicity against mouse J774 cells by MTT assay Cytotoxicity against mouse J774 cells by MTT assay	[PMID: 17627828]
Lymphocyte	EC₅₀	>500 nM Compound: alpha-TOH	Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis	[PMID: 33214825]
MCF7	IC₅₀	>300 μM Compound: alpha-TOH	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]
MCF7	IC₅₀	329 μM Compound: 1 (alpha-TOH)	Compound was tested for antiproliferative activity against human breast cancer cell line MCF-7 in MTT assay Compound was tested for antiproliferative activity against human breast cancer cell line MCF-7 in MTT assay	[PMID: 9873556]
MT4	IC₅₀	165 μM Compound: alpha-TOH	Cytotoxicity against human MT4 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MT4 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]
RPMI-8226	IC₅₀	>300 μM Compound: alpha-TOH	Cytotoxicity against human RPMI8226 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human RPMI8226 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]
THP-1	IC₅₀	>500 μM Compound: 1	Cytotoxicity against human THP1 cells by MTT assay Cytotoxicity against human THP1 cells by MTT assay	[PMID: 17627828]
U-937	IC₅₀	100 μM Compound: alpha-TOH	Cytotoxicity against human U937 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human U937 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]

In Vitro

α-Vitamin E ((+)-α-Tocopherol) is a peroxyl radical scavenger. The importance of this function is to maintain the integrity of long-chain polyunsaturated fatty acids in the membranes of cells and thus maintain their bioactivity^[1].
α-Vitamin E ((+)-α-Tocopherol) has been described to inhibit PKC in various cell types with consequent inhibition of platelet aggregation, endothelial cell nitric oxide production and superoxide production in neutrophils and macrophages. α-Vitamin E ((+)-α-Tocopherol) exposure induced the activation of both the MAP kinase and PI3 kinase (PI3K) pathways, suggesting that it is the oxidative stress that up-regulates kinase pathways and the antioxidant action of α-tocopherol protects the cell membrane fatty acids^[1].
α-Vitamin E ((+)-α-Tocopherol) has proposed benefits for influenza virus A infection, as well as possible activity against hepatitis B and C. α-Vitamin E shows proviral effects, particularly in HEK293T/17 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

α-Vitamin E ((+)-α-Tocopherol) prevents the increase in the pro-inflammatory cytokines IL-1, IL-6, and IFN-γ mRNA and protein compared with the ischemic-reperfused myocardium from untreated pigs and compared to the non-injured area^[1].
α-Vitamin E (D-α-Tocopherol; intraperitoneal injection or oral administration) treatment induces an amelioration of diabetic nephropathy in mice through the activation of diacylglycerol kinase α (DGKα) and the prevention of podocyte loss^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 59-02-9
Appearance Liquid (Density: 0.95 g/cm³)
Molecular Weight 430.71
Formula C₂₉H₅₀O₂
Color Colorless to light yellow
SMILES

OC1=C(C)C(C)=C2C(CC[C@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)O2)=C1C
Synonyms

(+)-α-Tocopherol; D-α-Tocopherol
Structure Classification
- Phenols
- Monophenols
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Publications (22)

All Flow CytometryWBCell Proliferation/Viability AssayRT-PCRIn Vivo Efficacy Study

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Journal Impact Factor

Signal Transduct Target Ther

Disruption of heme homeostasis by nuclear receptor Nur77 induces pyroptosis through granzyme B-dependent GSDMC cleavage. [Abstract]

2025 Dec 17;10(1):413. PMID: 41407678
Nat Nanotechnol

Intrinsic bioactivity of black phosphorus nanomaterials on mitotic centrosome destabilization through suppression of PLK1 kinase. [Abstract]

2021 Oct;16(10):1150-1160. PMID: 34354264
Nat Commun

Farnesoid X receptor activation by bile acids suppresses lipid peroxidation and ferroptosis. [Abstract]

2023 Oct 30;14(1):6908. PMID: 37903763
Acta Pharm Sin B

2026 Mar 3.
Redox Biol

Dissecting the process of human neutrophil lineage determination by using alpha-lipoic acid inducing neutrophil deficiency model. [Abstract]

2022 Aug;54:102392. PMID: 35797799

α-Vitamin E purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Aug;54:102392. [Abstract]

α-Vitamin E (25 mM; 7 days). Percentages of CD11b+ CD66b+ CD15+ CD14– neutrophils and CD11b+ CD14+ CD66b– CD15– monocytes derived from cord blood CD34+ hematopoietic stem/progenitor cells (HSPCs) treated with different antioxidants were measured.
Cell Rep Med

A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML. [Abstract]

2024 May 29:101592. PMID: 38843841
Cell Death Dis

Selenomethionine as a dual-mechanism ferroptosis inhibitor: selenium-supply-driven GPX4 biosynthesis beyond transsulfuration and reductive-capacity-mediated ROS scavenging independent of GPX4 activity. [Abstract]

2026 Feb 14;17(1):224. PMID: 41688429
Dev Cell

Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation. [Abstract]

2025 Apr 18:S1534-5807(25)00206-0. PMID: 40280135
Int J Biol Macromol

Polygonatum sibiricum polysaccharide attenuates cyclophosphamide-induced testicular damages and sperm defects in male mice via Nrf2 mediating antioxidant protective mechanisms. [Abstract]

2025 May;307(Pt 3):141968. PMID: 40081692
Biomed Pharmacother

Alpha-tocopherol inhibits ferroptosis and promotes neural function recovery in rats with spinal cord injury via downregulating Alox15. [Abstract]

2024 Jun:175:116734. PMID: 38754264
Antioxidants (Basel)

Alpha-Tocopherol Protects Porcine Oocytes from Acetamiprid-Induced Meiotic Defects by Alleviating Oxidative Stress-Mediated Ferroptosis. [Abstract]

2025 Oct 30;14(11):1304. PMID: 41300461
Antioxid Redox Signal

α-Tocopherol Ameliorates Liver Fibrosis by Inhibiting Hepatic Stellate Cell Activation by Promoting Nrf2 Nuclear Translocation. [Abstract]

2025 Aug 1. PMID: 40762285
Int J Mol Sci

Transcriptomic and Functional Analyses Reveal the Different Roles of Vitamins C, E, and K in Regulating Viral Infections in Maize. [Abstract]

2023 Apr 28;24(9):8012. PMID: 37175719

α-Vitamin E purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2023 Apr 28;24(9):8012. [Abstract]

The accumulations of MCMV CP proteins in MCMV- or S + M-infected B73 maize leaves at 9 dpi were detected by spraying vitamin E (1 mM) or K and Western blot analyses.

α-Vitamin E purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2023 Apr 28;24(9):8012. [Abstract]

The accumulations of SCMV RNA in MCMV- or S + M-infected maize leaves at 9 dpi after spraying Vitamin E (1 mM) or K were measured by RT–qPCR.
Molecules

Antiproliferation Activity and Mechanism of c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 on Colon Cancer Cells. [Abstract]

2020 Mar 9;25(5):1225. PMID: 32182796
Heliyon

Study on the molecular mechanisms of Liuwei Dihuang decoction against aging-related cognitive impairment based on network pharmacology and experimental verification. [Abstract]

2024 Jun 6;10(11):e32526. PMID: 38961903

α-Vitamin E purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Jun 6;10(11):e32526. [Abstract]

α-Vitamin E (50 μg/mL; 12 h). Comparison of the cell inhibition rate after administration was performed.

α-Vitamin E purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Jun 6;10(11):e32526. [Abstract]

Sixty mice were randomly divided into 5 groups after adaptive feeding for 7 days: control group, model group, Vit-E (200 mg/kg) group. LW alleviated cognitive dysfunction in aging mice. Navigation test.
Toxicol Appl Pharmacol

Dendrobine protects HACAT cells from H2O2-induced oxidative stress and apoptosis damage via Nrf2/Keap1/ARE signaling pathway. [Abstract]

2022 Nov 1:454:116217. PMID: 36058301
Biol Reprod

Astaxanthin promotes in vitro maturation of mouse oocytes by regulating mitochondrial functions and protein homeostasis. [Abstract]

2025 May 5:ioaf107. PMID: 40324205
Breast Cancer Res Treat

ZEB1 directly inhibits GPX4 transcription contributing to ROS accumulation in breast cancer cells. [Abstract]

2021 Jul;188(2):329-342. PMID: 34169392
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Animals

Impaired Mitochondrial Function Results from Oxidative Stress in the Full-Term Placenta of Sows with Excessive Back-Fat. [Abstract]

2020 Feb 23;10(2):360. PMID: 32102192
Transl Cancer Res

TAF1 inhibitor Bay-299 induces cell death in acute myeloid leukemia. [Abstract]

2021 Dec;10(12):5307-5318. PMID: 35116379
J Afr Ass Physiol Sci

7(1): 30-37, July 2019.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (232.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : ≥ 100 mg/mL (232.17 mM)

H₂O : < 0.1 mg/mL (insoluble)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3217 mL

11.6087 mL

23.2175 mL

5 mM

0.4643 mL

2.3217 mL

4.6435 mL

10 mM

0.2322 mL

1.1609 mL

2.3217 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 90% Corn Oil

Solubility: ≥ 5 mg/mL (11.61 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Maret G Traber, et al. Vitamin E, antioxidant and nothing more. Free Radic Biol Med. 2007 Jul 1;43(1):4-15. [Content Brief]

[2]. Daiki Hayashi, et al. Amelioration of diabetic nephropathy by oral administration of d-α-tocopherol and its mechanisms. Biosci Biotechnol Biochem. 2018 Jan;82(1):65-73. [Content Brief]

[3]. Atchara Paemanee, et al. Screening of melatonin, α-tocopherol, folic acid, acetyl-L-carnitine and resveratrol for anti-dengue 2 virus activity. BMC Res Notes. 2018 May 16;11(1):307. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / Ethanol	1 mM	2.3217 mL	11.6087 mL	23.2175 mL	58.0437 mL
	5 mM	0.4643 mL	2.3217 mL	4.6435 mL	11.6087 mL
	10 mM	0.2322 mL	1.1609 mL	2.3217 mL	5.8044 mL
	15 mM	0.1548 mL	0.7739 mL	1.5478 mL	3.8696 mL
	20 mM	0.1161 mL	0.5804 mL	1.1609 mL	2.9022 mL
	25 mM	0.0929 mL	0.4643 mL	0.9287 mL	2.3217 mL
	30 mM	0.0774 mL	0.3870 mL	0.7739 mL	1.9348 mL
	40 mM	0.0580 mL	0.2902 mL	0.5804 mL	1.4511 mL
	50 mM	0.0464 mL	0.2322 mL	0.4643 mL	1.1609 mL
	60 mM	0.0387 mL	0.1935 mL	0.3870 mL	0.9674 mL
	80 mM	0.0290 mL	0.1451 mL	0.2902 mL	0.7255 mL
	100 mM	0.0232 mL	0.1161 mL	0.2322 mL	0.5804 mL

Keywords

α-Vitamin E

22 Cited Publications

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Publications Citing Use of MedChemExpress (MCE) α-Vitamin E

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (22)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

α-Vitamin E Related Classifications

HY-N0683 Related Classifications