Nervous necrosis virus B2 non-structural protein shut off host translation via sequestration of polyadenylate-binding protein

  • Biochem Biophys Res Commun. 2026 Aug 20:827:154020. doi: 10.1016/j.bbrc.2026.154020.
Vertika Bajpai  1 ,  I-Wen Lin  2 ,  Ming-Hsien Chiang  2 ,  Chen-Hung Li  3 ,  Chien-Wen Chen  3 ,  Chia-Ching Chang  4 ,  Chao-An Cheng  5 ,  Yung-Song Wang  6 ,  Shih-Chieh Chen  7 ,  Chi-Yao Chang  8
Affiliations
  • 1. Institute of Fisheries Science, College of Life Science, National Taiwan University, Taipei, Taiwan; Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
  • 2. Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan; Department of Food Science, National Quemoy University, Kinmen, Taiwan.
  • 3. Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
  • 4. Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, Taiwan.
  • 5. Department of Food Science, National Quemoy University, Kinmen, Taiwan.
  • 6. Institute of Fisheries Science, College of Life Science, National Taiwan University, Taipei, Taiwan.
  • 7. School of Medicine, College of Life Sciences and Medicine, National Tsing Hua University, Hsinchu, Taiwan. Electronic address: [email protected].
  • 8. Institute of Fisheries Science, College of Life Science, National Taiwan University, Taipei, Taiwan; Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan; Department of Life Science, Fu-Jen Catholic University, New Taipei, Taiwan. Electronic address: [email protected].
PMID: 42218873 DOI: 10.1016/j.bbrc.2026.154020
Abstract

Nervous necrosis virus (NNV) is the causative agent of viral nervous necrosis disease in piscine larvae worldwide. As a positive-sense RNA virus, NNV performs its replication, transcription, translation and assembly in the cytoplasm of host cell. However, the ectopic expression of viral B2 non-structural protein was observed to translocate into nucleus of grouper brain cells. In this study, we found that the nuclear translocation of B2 was accompanied by host translation shutoff which was examined by the surface sensing of translation (SUnSET) puromycin labelling experiment. Furthermore, the ectopically expressed B2 protein was also observed to co-localize with polyadenylate-binding protein (PABP) in cytoplasm and nucleus of transfected cell. The far-Western blotting further confirmed the interaction between B2 and PABP. Finally, the decrease in B2 protein levels at the late stage of Infection appears to result from lysosome-mediated degradation facilitated by its interaction with Mx proteins. These results reveal that NNV B2 non-structural protein contributes to host translation shutoff after Infection.

Keywords
B2; Lysosome; Mx; Nervous necrosis virus; Polyadenylate-binding protein; Translation shutoff.
Products