Lactobacillus crispatus-derived exopolysaccharides with antibacterial activity limit Salmonella typhimurium invasion by inhibiting inflammasome-mediated pyroptosis

  • Food Funct. 2022 Sep 23. doi: 10.1039/d2fo02125k.
Chenchen Ding  1 ,  Huixian Wu  1  2 ,  Xiuyun Cao  1 ,  Zhangshan Gao  1 ,  Zhihui Tang  1 ,  Wentao Fan  1 ,  Liping Yan  1 ,  Bin Liu  2 ,  Hong Lin  3 ,  Suquan Song  1
Affiliations
  • 1. MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China. [email protected].
  • 2. Management Office of Dafeng Milu National Nature Reserve, Yancheng, 224136, China.
  • 3. Animal, Plant and Food Inspection Center, Nanjing Customs, Nanjing, 210019, China.
PMID: 36148688 DOI: 10.1039/d2fo02125k
Abstract

In this study, a novel heteropolysaccharide (EPS 7-4) with a molecular weight of 53 387 Da was isolated from Lactobacillus crispatus, and it was mainly composed of mannose (36.9%) and glucose (30.8%). EPS 7-4 showed excellent inhibitory effects on the proliferation, biofilm formation, and virulence factor gene expression of Salmonella typhimurium (S. typhimurium) by disrupting the integrity of the Bacterial wall. Furthermore, EPS 7-4 can effectively restrict Bacterial translocation, upregulate the abundance of Lactobacillus spp. and Bifidobacterium spp., and alleviate the S. typhimurium induced severe inflammatory response in the intestinal tract of mice. Besides, we demonstrated that EPS 7-4 can protect mice by inhibiting S. typhimurium induced Pyroptosis, with the mechanism that EPS 7-4 affects ASC oligomerization during inflammasome-mediated Pyroptosis. Therefore, due to its excellent anti-bacterial and anti-inflammatory abilities, EPS 7-4 is a promising health regulator owing to its excellent Antibacterial and anti-inflammatory abilities.

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