Long non-coding RNA MALAT1 regulates cell proliferation and apoptosis via miR-135b-5p/GPNMB axis in Parkinson's disease cell model

  • Biol Res. 2021 Mar 16;54(1):10. doi: 10.1186/s40659-021-00332-8.
Kefeng Lv  1 ,  Yuhua Liu  2 ,  Yanbing Zheng  2 ,  Shaowen Dai  2 ,  Peifeng Yin  2 ,  Haifeng Miao  3
Affiliations
  • 1. Department of Neurology, Affiliated Dongguan People's Hospital, Southern Medical University (Dongguan People's Hospital), Guangdong Province, 523059, Dongguan, People's Republic of China.
  • 2. Department of General Practice, Affiliated Dongguan People's Hospital, Southern Medical University (Dongguan People's Hospital), 3 South Wandao Road, Wanjiang District, 523059, Dongguan, Guangdong Province, People's Republic of China.
  • 3. Department of General Practice, Affiliated Dongguan People's Hospital, Southern Medical University (Dongguan People's Hospital), 3 South Wandao Road, Wanjiang District, 523059, Dongguan, Guangdong Province, People's Republic of China. [email protected].
PMID: 33726823 DOI: 10.1186/s40659-021-00332-8
Abstract

Backgrounds: Parkinson's Disease (PD) is a common age-related neurodegenerative disorder worldwide. This research aimed to investigate the effects and mechanism underlying long non-coding RNA metastasis-associated Lung Adenocarcinoma transcript 1 (MALAT1) in PD.

Methods: SK-N-SH and SK-N-BE cells were treated with MPP+ to establish the MPP+-stimulated cell model of PD, and MALAT1 expression was determined. Then, the effects of MALAT1 depletion on cell proliferation and Apoptosis were determined in the MPP+-stimulated cell model of PD. Besides, the correlations between microRNA-135b-5p (miR-135b-5p) and MALAT1 or glycoprotein nonmetastatic Melanoma protein B (GPNMB) in MPP+-stimulated cell model of PD were explored.

Results: MALAT1 was increasingly expressed and downregulation of MALAT1 promoted cell proliferation while inhibited Apoptosis in MPP+-stimulated cells. Besides, miR-135b-5p was a target of MALAT1 and directly targeted to GPNMB. Further investigation indicated that suppression of MALAT1 regulated cell proliferation and Apoptosis by miR-135b-5p/GPNMB axis.

Conclusion: Our findings reveal that MALAT1/miR-135b-5p/GPNMB axis regulated cell proliferation and Apoptosis in MPP+-stimulated cell model of PD, providing a potential biomarker and therapeutic target for PD.

Keywords
Apoptosis; Cell proliferation; GPNMB; MALAT1; Parkinson’s disease; miR-135b-5p.
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