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WWP2 Protein, Human (sf9, His-GST)

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The WWP2 protein is an E3 ubiquitin protein ligase that coordinates multiple ubiquitination events, including “Lys-63”-linked polyubiquitination of POU5F1, targeting it for proteasomal degradation in embryonic stem cells (ESCs). WWP2 also ubiquitinates EGR2, inhibits activation-induced cell death in T cells, and targets SLC11A2, which is enhanced by NDFIP1 and NDFIP2. WWP2 Protein, Human (sf9, His-GST) is the recombinant human-derived WWP2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for WWP2 Protein, Human (sf9, His-GST)
  • WWP2 Protein, Human (sf9, His-GST) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The WWP2 protein is an E3 ubiquitin protein ligase that coordinates multiple ubiquitination events, including “Lys-63”-linked polyubiquitination of POU5F1, targeting it for proteasomal degradation in embryonic stem cells (ESCs). WWP2 also ubiquitinates EGR2, inhibits activation-induced cell death in T cells, and targets SLC11A2, which is enhanced by NDFIP1 and NDFIP2. WWP2 Protein, Human (sf9, His-GST) is the recombinant human-derived WWP2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Background

WWP2 Protein, functioning as an E3 ubiquitin-protein ligase, facilitates the transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to its targeted substrates. Notably, WWP2 engages in the polyubiquitination of POU5F1 through 'Lys-63'-linked conjugation, leading to the proteasomal degradation of POU5F1. In embryonic stem cells (ESCs), this ubiquitination is proposed to play a regulatory role in controlling the protein levels of POU5F1. Additionally, WWP2 ubiquitinates EGR2, promoting its proteasomal degradation, and in T-cells, this ubiquitination is implicated in inhibiting activation-induced cell death. Moreover, WWP2 targets SLC11A2 for ubiquitination, a process enhanced in the presence of NDFIP1 and NDFIP2. Furthermore, WWP2 ubiquitinates RPB1, leading to its proteasomal degradation. These diverse ubiquitination events underscore the regulatory versatility of WWP2 in modulating the stability and abundance of key proteins involved in various cellular processes.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 85%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-His;N-GST
  • Accession

    O00308-1 (M1-E870)

  • Gene ID
    11060  [NCBI]
  • Molecular Construction
    • N-term
    • His-GST
    • WWP2 (M1-E870)
      Accession # O00308-1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    WWP2; WW Domain Containing E3 Ubiquitin Protein Ligase 2; AIP2; NEDD4-Like E3 Ubiquitin-Protein Ligase WWP2; HECT-Type E3 Ubiquitin Transferase WWP2; Atrophin-1-Interacting Protein 2; Atrophin-1 Interacting Protein 2; WW Domain-Containing Protein 2; WWP2-

  • AA Sequence

    MASASSSRAGVALPFEKSQLTLKVVSAKPKVHNRQPRINSYVEVAVDGLPSETKKTGKRIGSSELLWNEIIILNVTAQSHLDLKVWSCHTLRNELLGTASVNLSNVLKNNGGKMENMQLTLNLQTENKGSVVSGGELTIFLDGPTVDLGNVPNGSALTDGSQLPSRDSSGTAVAPENRHQPPSTNCFGGRSRTHRHSGASARTTPATGEQSPGARSRHRQPVKNSGHSGLANGTVNDEPTTATDPEEPSVVGVTSPPAAPLSVTPNPNTTSLPAPATPAEGEEPSTSGTQQLPAAAQAPDALPAGWEQRELPNGRVYYVDHNTKTTTWERPLPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQRNQLQGAMQHFSQRFLYQSSSASTDHDPLGPLPPGWEKRQDNGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESGTKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELYFIQDMEILGKVTTHELKEGGESIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKSTIYRHYTKNSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKSYEQLREKLLYAIEETEGFGQE

  • Predicted Molecular Mass

    126.7 kDa

  • Molecular Weight

    Approximately 120 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 85%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% Glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 85%, as determined by reducing SDS-PAGE.

  • WWP2 Protein, Human (sf9, His-GST) Product Image
    1/16
  • Experimental Validation Results for WWP2 Protein, Human (sf9, His-GST)
  • WWP2 Protein, Human (sf9, His-GST) Product Image