SMURF2 Protein, Human (His)

SMURF2 protein is an E3 ubiquitin protein ligase that interacts with SMAD7 to induce ubiquitin-dependent degradation of TGF-β receptors and downregulate TGF-β signaling. This interaction triggers autocatalytic degradation of SMURF2 and is antagonized by AIMP1. SMURF2 Protein, Human (His) is the recombinant human-derived SMURF2 protein, expressed by E. coli , with N-6*His labeled tag.

SMURF2 Protein, functioning as an E3 ubiquitin-protein ligase, operates by accepting ubiquitin from an E2 ubiquitin-conjugating enzyme and subsequently transferring ubiquitin directly to its targeted substrates. Notably, it interacts with SMAD7 to instigate SMAD7-mediated ubiquitin-dependent degradation of the transforming growth factor beta (TGF-beta) receptor, leading to the down-regulation of TGF-beta signaling. This interaction with SMAD7 not only triggers the degradation of the TGF-beta receptor but also activates SMURF2's autocatalytic degradation, a process antagonized by the association with AIMP1. Additionally, SMURF2 forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2, and SMAD3, targeting SMAD1 and SMAD2 for ubiquitination and subsequent degradation. In the context of filovirus infections such as Ebola/EBOV and Marburg/MARV, SMURF2 collaborates with the viral matrix protein VP40 to facilitate virus budding. Furthermore, SMURF2 plays a critical role in negatively regulating TGFB1-induced processes, including epithelial-mesenchymal transition and myofibroblast differentiation. This multifaceted functionality underscores the diverse regulatory roles of SMURF2 in cellular signaling and responses to infections.

Human

E. coli

N-6*His

Q9HAU4 (S2-E748)

64750

Partial

SNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

88.2 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 500 mM NaCl, 1 mM DTT, 20% glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.