NEDD4L Protein, Human (His)

The NEDD4L protein is an E3 ubiquitin protein ligase that regulates multiple signaling pathways, including autophagy, innate immunity, and DNA repair. NEDD4L Protein, Human (His) is the recombinant human-derived NEDD4L protein, expressed by E. coli , with N-6*His labeled tag. The total length of NEDD4L Protein, Human (His) is 974 a.a., .

NEDD4L, an E3 ubiquitin-protein ligase, exerts regulatory control over diverse cellular processes, including autophagy, innate immunity, and DNA repair. It negatively modulates TGF-beta signaling by inducing ubiquitination and proteasome-dependent degradation of SMAD2 and TGFBR1. In the context of autophagy, NEDD4L downregulates cell growth by ubiquitinating and reducing cellular levels of ULK1 or ASCT2. Additionally, it facilitates the ubiquitination and internalization of various plasma membrane channels, such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, Kv7.2, Kv7.3, and CLC5. NEDD4L also regulates the protein levels of SGK1, TNK2, BRAT1, and NTRK1, with its ubiquitin ligase activity influenced by NDFIP1. Furthermore, NEDD4L plays a crucial role in dendrite formation by melanocytes, contributes to TOR signaling regulation, and participates in antiviral innate immunity by catalyzing 'Lys-29'-linked cysteine ubiquitination of TRAF3, enhancing 'Lys-48' and 'Lys-63'-linked ubiquitination of TRAF3.

Human

E. coli

N-6*His

Q96PU5 (A2-D975)

23327

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.