NEDD4L Protein, Human
Based on 1 Customer Validation
The NEDD4L protein is an E3 ubiquitin protein ligase that regulates multiple signaling pathways, including autophagy, innate immunity, and DNA repair. NEDD4L Protein, Human is the recombinant human-derived NEDD4L protein, expressed by E. coli , with tag free.
- Species: Human
- Source: E. coli
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
The NEDD4L protein is an E3 ubiquitin protein ligase that regulates multiple signaling pathways, including autophagy, innate immunity, and DNA repair. NEDD4L Protein, Human is the recombinant human-derived NEDD4L protein, expressed by E. coli , with tag free.
NEDD4L, an E3 ubiquitin-protein ligase, exerts regulatory control over diverse cellular processes, including autophagy, innate immunity, and DNA repair. It negatively modulates TGF-beta signaling by inducing ubiquitination and proteasome-dependent degradation of SMAD2 and TGFBR1. In the context of autophagy, NEDD4L downregulates cell growth by ubiquitinating and reducing cellular levels of ULK1 or ASCT2. Additionally, it facilitates the ubiquitination and internalization of various plasma membrane channels, such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, Kv7.2, Kv7.3, and CLC5. NEDD4L also regulates the protein levels of SGK1, TNK2, BRAT1, and NTRK1, with its ubiquitin ligase activity influenced by NDFIP1. Furthermore, NEDD4L plays a crucial role in dendrite formation by melanocytes, contributes to TOR signaling regulation, and participates in antiviral innate immunity by catalyzing 'Lys-29'-linked cysteine ubiquitination of TRAF3, enhancing 'Lys-48' and 'Lys-63'-linked ubiquitination of TRAF3.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag Tag Free
-
Accession
Q96PU5 (A2-D975)
-
Gene ID23327
-
Molecular Construction
-
N-term
-
NEDD4L (A2-D975)
Accession # Q96PU5 -
C-term
-
-
Protein Length
Full Length of Mature Protein
-
Synonyms
NEDD4L; Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like; NEDD4 Like E3 Ubiquitin Protein Ligase; HECT-Type E3 Ubiquitin Transferase; HECT-Type E3 Ubiquitin Transferase NED4L; Ubiquitin-Protein Ligase Rsp5; E3 Ubiquitin-Protein Ligas
-
AA Sequence
ATGLGEPVYGLSEDEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSDQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSAPAGRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD
-
Predicted Molecular Mass
111.9 kDa
-
Molecular Weight
Approximately 95-100 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
-
Data Sheet (240 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
