ITCH/AIP4 Protein, Human

ITCH/AIP4 protein is an E3 ubiquitin protein ligase that catalyzes the conjugation of “Lys-29”, “Lys-48” and “Lys-63” linked ubiquitin, affecting inflammatory signaling pathways. It forms a ubiquitin editing complex that promotes RIPK1 degradation and terminates TNF- or LPS-mediated NFKB1 activation. ITCH/AIP4 Protein, Human is the recombinant human-derived ITCH/AIP4 protein, expressed by E. coli , with tag free.

ITCH/AIP4 functions as an E3 ubiquitin-protein ligase, orchestrating the transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, catalyzing 'Lys-29,' 'Lys-48,' and 'Lys-63'-linked ubiquitin conjugation. It plays a crucial role in the regulation of inflammatory signaling pathways, forming an integral part of a ubiquitin-editing protein complex that modulates the transient nature of inflammatory responses. The complex, including TNFAIP3, TAX1BP1, and RNF11, ensures the termination of TNF- or LPS-mediated activation of NFKB1 by deubiquitinating 'Lys-63' polyubiquitin chains on RIPK1 and catalyzing the formation of 'Lys-48'-polyubiquitin chains. ITCH/AIP4 also ubiquitinates various proteins involved in immune response regulation, transcriptional activity modulation, and cellular antiviral responses. Notably, it participates in the degradation of proteins such as JUN, JUNB, and MAVS, highlighting its diverse roles in cellular processes, including apoptosis, reactive oxygen species regulation, and antiviral defense.

Measured by its binding ability in a functional ELISA. Immobilized Human ITCH at 2 μg/mL (100 μL/well) can bind Human LDLRAD3 (HY-P77057A). The ED₅₀ for this effect is 1.13 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human ITCH at 2 μg/mL (100 μL/well) can bind Human LDLRAD3 (HY-P77057A). The ED₅₀ for this effect is 1.13 μg/mL.

Human

E. coli

Tag Free

Q96J02-2/NP_113671.3 (R485-E862)

83737  [NCBI]

Partial

RDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Approximately 45 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, 200 mM L-Arginine, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.