ITCH/AIP4 Protein, Human

ITCH/AIP4 Protein, an E3 ubiquitin-protein ligase, catalyzes 'Lys-29,' 'Lys-48,' and 'Lys-63'-linked ubiquitin conjugation, influencing inflammatory signaling pathways. It forms a ubiquitin-editing complex, promoting RIPK1 degradation and termination of TNF- or LPS-mediated NFKB1 activation. It regulates transcription factors, immune response, and hematopoietic lineage development. ITCH/AIP4 targets JUN, JUNB, DTX1, and MAVS for degradation, modulates Wnt receptor sorting, and controls CXCR4 trafficking. It also regulates apoptosis, reactive oxygen species levels, and inhibits influenza A virus replication. ITCH/AIP4 Protein, Human is the recombinant human-derived ITCH/AIP4 protein, expressed by E. coli , with tag free. The total length of ITCH/AIP4 Protein, Human is 378 a.a., with molecular weight of ~45 kDa.

ITCH/AIP4 functions as an E3 ubiquitin-protein ligase, orchestrating the transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, catalyzing 'Lys-29,' 'Lys-48,' and 'Lys-63'-linked ubiquitin conjugation. It plays a crucial role in the regulation of inflammatory signaling pathways, forming an integral part of a ubiquitin-editing protein complex that modulates the transient nature of inflammatory responses. The complex, including TNFAIP3, TAX1BP1, and RNF11, ensures the termination of TNF- or LPS-mediated activation of NFKB1 by deubiquitinating 'Lys-63' polyubiquitin chains on RIPK1 and catalyzing the formation of 'Lys-48'-polyubiquitin chains. ITCH/AIP4 also ubiquitinates various proteins involved in immune response regulation, transcriptional activity modulation, and cellular antiviral responses. Notably, it participates in the degradation of proteins such as JUN, JUNB, and MAVS, highlighting its diverse roles in cellular processes, including apoptosis, reactive oxygen species regulation, and antiviral defense.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

Tag Free

Q96J02-2/NP_113671.3 (R485-E862)

83737  [NCBI]

Approximately 45 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.