1. Recombinant Proteins
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Transferases (EC 2) Ubiquitin Enzymes
  4. E3 Ligases
  5. RNF114 Protein, Human

RNF114 Protein, Human

Cat. No.: HY-P701548
COA Handling Instructions

The RNF114 protein is an E3 ubiquitin protein ligase that critically regulates a variety of cellular processes by ubiquitinating various substrates. RNF114 is involved in cell cycle progression, apoptosis, osteoclastogenesis and immunity, serving as a negative regulator of NF-kappa-B-dependent transcription, affecting immune responses by ubiquitinating TNFAIP3 and potentially TRAF6. RNF114 Protein, Human is the recombinant human-derived RNF114 protein, expressed by E. coli , with tag free. The total length of RNF114 Protein, Human is 227 a.a., .

For research use only. We do not sell to patients.

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Size Price Stock Quantity
5 μg $115 In-stock
10 μg $200 In-stock
50 μg $540 In-stock
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Description

The RNF114 protein is an E3 ubiquitin protein ligase that critically regulates a variety of cellular processes by ubiquitinating various substrates. RNF114 is involved in cell cycle progression, apoptosis, osteoclastogenesis and immunity, serving as a negative regulator of NF-kappa-B-dependent transcription, affecting immune responses by ubiquitinating TNFAIP3 and potentially TRAF6. RNF114 Protein, Human is the recombinant human-derived RNF114 protein, expressed by E. coli , with tag free. The total length of RNF114 Protein, Human is 227 a.a., .

Background

RNF114 Protein, functioning as an E3 ubiquitin-protein ligase, plays a pivotal role in orchestrating diverse cellular processes by promoting the ubiquitination of various substrates. With broad implications, it is involved in the regulation of fundamental biological processes including cell cycle progression, apoptosis, osteoclastogenesis, as well as both innate and adaptive immunity. RNF114 acts as a negative regulator of NF-kappa-B-dependent transcription by facilitating the ubiquitination and stabilization of the NF-kappa-B inhibitor TNFAIP3, and potentially TRAF6, thereby influencing immune responses. Additionally, it functions as a negative regulator of T-cell activation and impedes cellular dsRNA responses and interferon production by targeting the MAVS component for proteasomal degradation. Through its ubiquitination of CDK inhibitors, such as CDKN1A, CDKN1B, and CDKN1C, RNF114 stimulates G1-to-S phase transition, suppressing cellular senescence and potentially contributing to spermatogenesis. This multifaceted activity underscores the significance of RNF114 in governing key cellular pathways and processes.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

Q9Y508 (A2-Q228)

Gene ID

55905

Molecular Construction
N-term
RNF114 (A2-Q228)
Accession # Q9Y508
C-term
Synonyms
RNF114; E3 ubiquitin-protein ligase RNF114; RING finger protein 114; RING-type E3 ubiquitin transferase RNF114; Zinc finger protein 228; Zinc finger protein 313
Molecular Weight

Approximately 25.7 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 200 mM NaCl, 5% glycerol, 1 mM DTT, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
RNF114 Protein, Human
Cat. No.:
HY-P701548
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