RNF43 Protein, Mouse (HEK293, Fc)

RNF43 Protein, a receptor tyrosine kinase, regulates the Wnt signaling pathway by promoting the degradation of Frizzled receptors. It plays a crucial role in embryonic development and tissue homeostasis. Dysregulation of RNF43 Protein is associated with various cancers, making it a potential therapeutic target in cancer treatment. RNF43 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived RNF43 protein, expressed by HEK293 , with C-hFc labeled tag.

RNF43 is an E3 ubiquitin-protein ligase with a pivotal role as a negative regulator in the Wnt signaling pathway. It functions by facilitating the ubiquitination, endocytosis, and subsequent degradation of components in the Wnt receptor complex, specifically targeting Frizzled. Its regulatory influence extends to both canonical and non-canonical Wnt signaling pathways, as demonstrated in various studies. Additionally, in conjunction with RSPO2 and ZNRF3, RNF43 forms a crucial regulatory triad, serving as a master switch that governs limb specification. This underscores the significance of RNF43 in orchestrating key developmental processes and fine-tuning Wnt signaling for proper cellular functions.

Immobilized Mouse R spondin 1, His Tag at 2 μg/mL (100 μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC₅₀ of ≤0.21 μg/mL determined by ELISA.

Mouse

HEK293

C-hFc

Q5NCP0-1 (G24-Y197)

207742  [NCBI]

Extracellular Domain

GFGHTGRVLAAAVESERSAEQKAVIRVIPLKMDPTGKLNLTLEGVFAGVAEVTPAEGKLMQSHPLYLCNASDDDNLEPGFISIVKLESPRRAPRPCLSLASKARMAGERGANAVLFDITEDRSAAEQLQQPLGLTKPVVLIWGSDAAKLMEFVYKNRKAYVWIELKEPPAGANY

53-60 kDa

≥ 95%, as determined by Bis-Tris PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.