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RNF43 Protein, Mouse (HEK293, Fc)

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RNF43 Protein, a receptor tyrosine kinase, regulates the Wnt signaling pathway by promoting the degradation of Frizzled receptors. It plays a crucial role in embryonic development and tissue homeostasis. Dysregulation of RNF43 Protein is associated with various cancers, making it a potential therapeutic target in cancer treatment. RNF43 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived RNF43 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

RNF43 Protein, a receptor tyrosine kinase, regulates the Wnt signaling pathway by promoting the degradation of Frizzled receptors. It plays a crucial role in embryonic development and tissue homeostasis. Dysregulation of RNF43 Protein is associated with various cancers, making it a potential therapeutic target in cancer treatment. RNF43 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived RNF43 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

RNF43 is an E3 ubiquitin-protein ligase with a pivotal role as a negative regulator in the Wnt signaling pathway. It functions by facilitating the ubiquitination, endocytosis, and subsequent degradation of components in the Wnt receptor complex, specifically targeting Frizzled. Its regulatory influence extends to both canonical and non-canonical Wnt signaling pathways, as demonstrated in various studies. Additionally, in conjunction with RSPO2 and ZNRF3, RNF43 forms a crucial regulatory triad, serving as a master switch that governs limb specification. This underscores the significance of RNF43 in orchestrating key developmental processes and fine-tuning Wnt signaling for proper cellular functions.

Verified Bioactivity

Immobilized Mouse R spondin 1, His Tag at 2 μg/mL (100 μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of ≤0.21 μg/mL determined by ELISA.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-hFc
  • Accession

    Q5NCP0-1 (G24-Y197)

  • Gene ID
    207742  [NCBI]
  • Molecular Construction
    • N-term
    • RNF43 (G24-Y197)
      Accession # Q5NCP0-1
    • hFc
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    RNF43; DKFZp781H0392; Ring Finger Protein 43; FLJ20315; RNF124; RING-Type E3 Ubiquitin Transferase; URCC; RING Finger Protein 43; RING-Type E3 Ubiquitin Transferase RNF43; SSPCS; E3 Ubiquitin-Protein Ligase RNF43

  • AA Sequence

    GFGHTGRVLAAAVESERSAEQKAVIRVIPLKMDPTGKLNLTLEGVFAGVAEVTPAEGKLMQSHPLYLCNASDDDNLEPGFISIVKLESPRRAPRPCLSLASKARMAGERGANAVLFDITEDRSAAEQLQQPLGLTKPVVLIWGSDAAKLMEFVYKNRKAYVWIELKEPPAGANY

  • Predicted Molecular Mass

    45.6 kDa

  • Molecular Weight

    Approximately 53-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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