RNF43 Protein, Human (HEK293, Fc)
Based on 1 Customer Validation
RNF43 is an E3 ubiquitin protein ligase and a key negative regulator in the Wnt signaling pathway. Its role involves mediating the ubiquitination, endocytosis, and subsequent degradation of components within the Wnt receptor complex, particularly Frizzled. RNF43 Protein, Human (HEK293, Fc) is the recombinant human-derived RNF43 protein, expressed by HEK293 , with C-hFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
RNF43 is an E3 ubiquitin protein ligase and a key negative regulator in the Wnt signaling pathway. Its role involves mediating the ubiquitination, endocytosis, and subsequent degradation of components within the Wnt receptor complex, particularly Frizzled. RNF43 Protein, Human (HEK293, Fc) is the recombinant human-derived RNF43 protein, expressed by HEK293 , with C-hFc labeled tag.
Background
RNF43, an E3 ubiquitin-protein ligase, serves as a critical negative regulator of the Wnt signaling pathway by orchestrating the ubiquitination, endocytosis, and subsequent degradation of key components in the Wnt receptor complex, specifically targeting Frizzled. This regulatory activity extends to both canonical and non-canonical Wnt signaling pathways. RNF43's role is underscored by its involvement as part of a master switch, alongside RSPO2 and ZNRF3, governing limb specification. This implies its significance in developmental processes and highlights its multifaceted involvement in modulating Wnt signaling, making it a crucial player in cellular and developmental pathways.
Verified Bioactivity
Immobilized Human RSPO3 His at 2 μg/mL (100 μL/well) can bind Human RNF43 hFc, the EC50 of Human RNF43 hFc is 3.0-12.0 ng/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-hFc
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Accession
Q68DV7-1 (G24-Y197)
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Molecular Construction
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N-term
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RNF43 (G24-Y197)
Accession # Q68DV7-1 -
hFc
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C-term
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Protein Length
Extracellular Domain
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Synonyms
RNF43; DKFZp781H0392; Ring Finger Protein 43; FLJ20315; RNF124; RING-Type E3 Ubiquitin Transferase; URCC; RING Finger Protein 43; RING-Type E3 Ubiquitin Transferase RNF43; SSPCS; E3 Ubiquitin-Protein Ligase RNF43
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AA Sequence
GFGRTGLVLAAAVESERSAEQKAIIRVIPLKMDPTGKLNLTLEGVFAGVAEITPAEGKLMQSHPLYLCNASDDDNLEPGFISIVKLESPRRAPRPCLSLASKARMAGERGASAVLFDITEDRAAAEQLQQPLGLTWPVVLIWGNDAEKLMEFVYKNQKAHVRIELKEPPAWPDY
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Predicted Molecular Mass
45.8 kDa
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Molecular Weight
Approximately 52 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (260 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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