Protein Deglycosylation Kit(for O-linked Glycans)
Based on 1 Customer Validation
Protein Deglycosylation Kit (for O-linked Glycans)contains O-Glycosidase and a2-3,6,8,9 Neuraminidase.Both enzymes are crucial tool enzymes for glycosylation research and are used in combination to enzymatically release O-linked glycans from glycoproteins.O-Glycosidase is capable of cleaving and releasing Core 1 (Gal-B(1-3)-GalNAc-)and Core 3(GIcNAc-B(1-3)-GalNAc-)O-linked disaccharides,which are linked to the hydroxyl groups of Ser or Thr residues in glycoproteins.a2-3,6,8,9 Neuraminidase can remove a(2,3)-,a(2,6)-,a(2,8)-,and a(2,9)-sialic acid residues from the non-reducing termini of O-linked glycans.
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Storage :
-20°C, 2 years.
Storage
-20°C, 2 years.
Components
| Components | HY-KE8012-1 kit |
|---|---|
| O-Glycosidase | 1200000 U ( 30 μL ) |
| α2-3,6,8,9 Neuraminidase | 0.6 U ( 30 μL ) |
| 10×Denaturing buffer | 1 mL |
| 10×Glyco buffer | 1 mL |
| 10% NP-40 | 1 mL |