BsmB I
BsmB I is one of the endonucleases of Type IIs that recognize non-palindromic sequences and cut outside of the recognition sequence, and is commonly used in Golden Gate assembly. Isoschizomers: Esp3 I, BstGZ53 I, Esp16 I, Esp23 I.
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Storage :
-20°C, 2 years.
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Description & Advantages
• BsmB I is one of the endonucleases of Type IIs that recognize non-palindromic sequences and cut outside of the recognition sequence, and is commonly used in Golden Gate assembly. Isoschizomers: Esp3 I, BstGZ53 I, Esp16 I, Esp23 I.
• Cleavage site:
5’…C G T C T C (N)1▼…3’
3’…G C A G A G (N)5▲…5’
Storage
-20°C, 2 years.
Components
| Components | HY-KE7050-200U | HY-KE7050-1000U |
|---|---|---|
| BsmB I (10 U/μL) | 20 μL | 100 μL |
| 10× BsmB I Buffer | 200 μL | 1 mL |
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