BsmB I

Cat. No.: HY-KE7050: FAQs
Manual SDS

BsmB I is one of the endonucleases of Type IIs that recognize non-palindromic sequences and cut outside of the recognition sequence, and is commonly used in Golden Gate assembly. Isoschizomers: Esp3 I, BstGZ53 I, Esp16 I, Esp23 I.

Size	Price	Stock	Quantity
200 U	USD 128	In-stock	Estimated Time of Arrival: December 31
1000 U	USD 456	In-stock	Estimated Time of Arrival: December 31

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BsmB I Featured Recommendations:

Description
Storage
Components
Documentation

Description
& Advantages

• Bsa I, ADCF is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. This product is animal derived component free, and has good redundancy of enzyme activity, which can easily cope with excessive substrate or difficult template enzyme cutting.

• Cleavage site:

5’…G G T C T C (N)₁^▼…3’

3’…C C A G A G (N)₅_▲…5’

Storage

-20°C, 2 years.

Components

Components	HY-KE7050-200U	HY-KE7050-1000U
BsmB I (10 U/μL)	20 μL	100 μL
10× BsmB I Buffer	200 μL	1 mL

Documentation

Manual (219 KB) SDS (105 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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