Protein Deglycosylation Kit (for N-linked & Complex O-linked Glycans)
Based on 1 Customer Validation
Protein Deglycosylation Kit (for N-linked & Complex O-linked Glycans) includes the enzymes and reagents re uired for the removal of all N-linked and some complex O-linked glycans. The enzyme reagent in this kit is a mixture of five glycosidases, namely PNGase F, O-Glycosidase, α2-3,6,8,9 Neuraminidase, β1-4 Galactosidase, and β-N-Acetylhexosaminidase. All enzymes in this kit are recombinant enzymes expressed in Escherichia coli BL21 and subseq uently purified. The molecular weights of these enzymes are as follows: PNGase F is 36 kDa, O-Glycosidase is 14 kDa,α2-3,6,8,9 Neuraminidase is 66 kDa, β1-4 Galactosidase is 94 kDa, and β-N-Acetylhexosaminidase is 55 kDa.
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Storage :
PNGaseF (Glycerol-free) should be stored at 4°C and not frozen. Valid for 2 years. All other components can be stored at -20°C and have a validity period of 2 years.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Storage
PNGaseF (Glycerol-free) should be stored at 4°C and not frozen. Valid for 2 years. All other components can be stored at -20°C and have a validity period of 2 years.
Components
| Cat. No. | Product List | Components | HY-KE8014-1 kit | Storage |
|---|---|---|---|---|
| HY-KE8014-A | Protein Deglycosylation Kit (for N-linked & Complex O-linked Glycans) reagent A | PNGase F (Glycerol-free) | 15000 U ( 30 μL ) | 4°C, 2 years. Do not freeze |
| HY-KE8014-B | Protein Deglycosylation Kit (for N-linked & Complex O-linked Glycans) reagent B | O-Glycosidase | 1200000 U ( 30 μL ) | -20°C, 2 years. |
| α2-3,6,8,9 Neuraminidase | 0.6 U ( 30 μL ) | |||
| β1-4 Galactosidase | 0.06 U ( 30 μL ) | |||
| β-N-acetylhexosaminidase | 1.5 U ( 30 μL ) | |||
| 10×Denaturing buffer | 1 mL | |||
| 10×Glyco buffer | 1 mL | |||
| 10% NP-40 | 1 mL |
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