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  3. Oxidative stress induced pyroptosis leads to osteogenic dysfunction of MG63 cells

Oxidative stress induced pyroptosis leads to osteogenic dysfunction of MG63 cells

  • J Mol Histol. 2020 Jun;51(3):221-232. doi: 10.1007/s10735-020-09874-9.
Shanshan Liu 1,  Juan Du 1 2,  Dongfang Li 1,  Panpan Yang 1,  Yuying Kou 1,  Congshan Li 1,  Qin Zhou 1,  Yupu Lu 1,  Tomoka Hasegawa 3,  Minqi Li 4
Affiliations

Affiliations

  • 1 Department of Bone Metabolism, School and Hospital of Stomatology, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, 250012, China.
  • 2 Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, China.
  • 3 Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, 060-8586, Japan.
  • 4 Department of Bone Metabolism, School and Hospital of Stomatology, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, 250012, China. [email protected].
PMID: 32356234 DOI: 10.1007/s10735-020-09874-9
Abstract

Periodontitis is characterized by alveolar bone destruction and is one of the most common chronic oral diseases. Inflammatory cytokines released by Pyroptosis, which can be triggered by oxidative stress, are critical in the development of periodontitis. This study aims to clarify whether oxidative stress causes osteoblast dysfunction by inducing Pyroptosis in the process of periodontitis. We found that treatment with lipopolysaccharide (LPS) led to NLRP3 inflammasome-mediated Pyroptosis of MG63 cells as well as decreased cell migration. Of note, LPS stimulation increased LDH release in a time- and dose-dependent manner. However, inhibition of Reactive Oxygen Species with N-acetyl-L-cysteine attenuated oxidative stress-mediated Pyroptosis and improved migration injury in osteoblasts treated with LPS. Further, inhibition of the NLRP3 inflammasome with MCC950 improved osteoblast migration and restored the expression of osteogenic differentiation-related proteins such as COL 1, RUNX 2 and ALP. In conclusion, oxidative stress caused by LPS induces Pyroptosis in osteoblasts, leading to osteogenic dysfunction.

Keywords

Migration; Mineralization; Osteoblast; Oxidative stress; Periodontitis; Pyroptosis.

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