X-Gluc Dicyclohexylamine

Cat. No.: HY-15935 Pureza: 99.40%: Ficha de datos
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X-Gluc Dicyclohexylamine is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system).

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X-Gluc Dicyclohexylamine Estructura química

No. CAS : 18656-96-7

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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This product is a controlled substance and not for sale in your territory.

Other Forms of X-Gluc Dicyclohexylamine:

X-Gluc cyclohexanamine In-stock
X-Gluc sodium In-stock

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•Related Small Molecules:

•Related Proteins:

Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolve 20 mg X-Gluc Dicyclohexylamine in DMSO to prepare X-Gluc master mix.
2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.
3. Allow the plates to air dry and be used to inoculate the organisms.
4. Incubate the plates at 35°C and observe 16-24 h after inoculation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

603.93

Fòrmula

C₂₆H₃₆BrClN₂O₇

No. CAS

18656-96-7

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]([C@@H](O)[C@@H]1O)[C@@H](O[C@@H]1C(O)=O)OC2=CNC3=C2C(Cl)=C(Br)C=C3.C4(CCCCC4)NC5CCCCC5

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvente y solubilidad

In Vitro:

DMSO : ≥ 30 mg/mL (49.67 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6558 mL	8.2791 mL	16.5582 mL
5 mM	0.3312 mL	1.6558 mL	3.3116 mL
10 mM	0.1656 mL	0.8279 mL	1.6558 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Pureza y Documentación

Purity: 99.40%

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Ficha de datos (280 KB) SDS (252 KB)

COA (260 KB) RP-HPLC (234 KB) MS (72 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6558 mL	8.2791 mL	16.5582 mL	41.3955 mL
	5 mM	0.3312 mL	1.6558 mL	3.3116 mL	8.2791 mL
	10 mM	0.1656 mL	0.8279 mL	1.6558 mL	4.1396 mL
	15 mM	0.1104 mL	0.5519 mL	1.1039 mL	2.7597 mL
	20 mM	0.0828 mL	0.4140 mL	0.8279 mL	2.0698 mL
	25 mM	0.0662 mL	0.3312 mL	0.6623 mL	1.6558 mL
	30 mM	0.0552 mL	0.2760 mL	0.5519 mL	1.3799 mL
	40 mM	0.0414 mL	0.2070 mL	0.4140 mL	1.0349 mL