X-Gluc sodium
Based on 1 Customer Validation
X-Gluc sodium is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue).
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 129541-41-9
- Formula: C14H12BrClNNaO7
- Molecular Weight:444.59
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolve 20 mg X-Gluc sodium in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix.
2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.
3. Allow the plates to air dry and be used to inoculate the organisms.
4. Incubate the plates at 35°C and observe 16-24 h after inoculation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 129541-41-9
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Appearance Solid
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Molecular Weight 444.59
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Formula C14H12BrClNNaO7
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Color White to off-white
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SMILES
O[C@H]([C@H]([C@@H]([C@@H](C(O[Na])=O)O1)O)O)[C@@H]1OC2=CNC3=C2C(Cl)=C(Br)C=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : ≥ 100 mg/mL (224.93 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2493 mL | 11.2463 mL | 22.4926 mL | 56.2316 mL |
| 5 mM | 0.4499 mL | 2.2493 mL | 4.4985 mL | 11.2463 mL | |
| 10 mM | 0.2249 mL | 1.1246 mL | 2.2493 mL | 5.6232 mL | |
| 15 mM | 0.1500 mL | 0.7498 mL | 1.4995 mL | 3.7488 mL | |
| 20 mM | 0.1125 mL | 0.5623 mL | 1.1246 mL | 2.8116 mL | |
| 25 mM | 0.0900 mL | 0.4499 mL | 0.8997 mL | 2.2493 mL | |
| 30 mM | 0.0750 mL | 0.3749 mL | 0.7498 mL | 1.8744 mL | |
| 40 mM | 0.0562 mL | 0.2812 mL | 0.5623 mL | 1.4058 mL | |
| 50 mM | 0.0450 mL | 0.2249 mL | 0.4499 mL | 1.1246 mL | |
| 60 mM | 0.0375 mL | 0.1874 mL | 0.3749 mL | 0.9372 mL | |
| 80 mM | 0.0281 mL | 0.1406 mL | 0.2812 mL | 0.7029 mL | |
| 100 mM | 0.0225 mL | 0.1125 mL | 0.2249 mL | 0.5623 mL |
X-Gluc sodium Related Classifications
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