β-glucuronidase
GUSB
β-glucuronidase is a glycoside hydrolase widely distributed in mammalian tissues, body fluids, microbiota, plants, fish, insects and mollusks. β-glucuronidase belongs to GH family 1, 2, 30, 79, 154 and GH-A. β-glucuronidase can hydrolyze estrogen glucuronides, regulate the enterohepatic circulation and reabsorption of estrogen, and affect estrogen metabolism. β-glucuronidase can also hydrolyze the extracellular sugar residues on the TRPV5 channel, trapping it in the plasma membrane, maintaining calcium channel activity and membrane calcium permeability. This target is related to many disease areas. For example, in cancer, its increased activity is related to the occurrence and development of colorectal cancer, breast cancer, ovarian cancer, etc.; in metabolic diseases, it is related to diabetes and its induced periodontitis; in inflammatory diseases, it involves bacterial peritonitis, bacterial meningitis, pelvic inflammatory disease, etc.; in addition, it is also related to diseases such as endometriosis[1][2][3][4][5][6][7].
References:
- [1]. Wei Y, et al. Gut dysbiosis-derived β-glucuronidase promotes the development of endometriosis. Fertil Steril. 2023 Sep;120(3 Pt 2):682-694. [Content Brief]
- [2]. Awolade P, et al. Therapeutic significance of β-glucuronidase activity and its inhibitors: A review. European Journal of Medicinal Chemistry, 2020, 187: 111921.
- [3]. Kim DH, et al. Intestinal bacterial beta-glucuronidase activity of patients with colon cancer. Arch Pharm Res. 2001 Dec;24(6):564-7. [Content Brief]
- [4]. Chang Q, et al. The beta-glucuronidase klotho hydrolyzes and activates the TRPV5 channel. Science. 2005 Oct 21;310(5747):490-3. [Content Brief]
- [5]. Sui Y, et al. The Role of Gut Microbial β-Glucuronidase in Estrogen Reactivation and Breast Cancer. Front Cell Dev Biol. 2021 Aug 12;9:631552. [Content Brief]
- [6]. Liu Z, et al. A novel allosteric driver mutation of β-glucuronidase promotes head and neck squamous cell carcinoma progression through STT3B-mediated PD-L1 N-glycosylation. MedComm (2020). 2025 Jan 19;6(2):e70062. [Content Brief]
- [7]. Paigen K. Mammalian beta-glucuronidase: genetics, molecular biology, and cell biology. Prog Nucleic Acid Res Mol Biol. 1989;37:155-205. [Content Brief]
All Product Categories
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β-glucuronidase Related Products (54)
Related Products (54)
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hCAII-IN-4
0 ImagesCat. No.: HY-150973CAS No.: 2816080-18-7hCAII-IN-4 (Compound 12j) is a potent hCA II inhibitor with an IC50 of 7.78 μM. hCAII-IN-4 also inhibits β-glucuronidase with an IC50 of 773.9 μM.
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Aceglatone
0 ImagesAceglatone, an antineoplastic agent, is a β-glucuronidase inhibitor. Aceglatone is a compound that inhibits colon carcinogenesis and prevents the induction of colon cancer by inhibiting the hydrolysis of glucuronides. Aceglatone reduces cell proliferation, induces apoptosis and inhibits DNA synthesis in human colon cancer cells.
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β-Glucuronidase-IN-6
0 ImagesCat. No.: HY-189055CAS No.: 3132205-57-0β-Glucuronidase-IN-6 is an Escherichia coli β-glucuronidase (EcGUS) inhibitor with an IC50 of 0.39 μM, Ki of 0.60 μM, selectivity over bovine liver β-glucuronidase, and oral effectiveness. β-Glucuronidase-IN-6 preferentially binds the enzyme-substrate complex, interacts with residues Asp163, Glu413, and Trp549 to stabilize the binding complex. β-Glucuronidase-IN-6 does not inhibit Escherichia coli growth and exhibits low cytotoxicity toward human epithelial cells. β-Glucuronidase-IN-6 attenuates irinotecan-induced weight loss, diarrhea, and colonic injury in mice. β-Glucuronidase-IN-6 can be used for the research of irinotecan-induced gastrointestinal toxicity.
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C12FDGlcU
0 ImagesCat. No.: HY-D1578CAS No.: 423762-14-5C12FDGlcU is a lipophilic analog of fluorescein di-β-D-glucuronic acid. C12FDGlcU can be useful for the detection of β-glucuronidase (GUS) gene expression. C12FDGlcU can enter the cells and then be cleaved by β-glucuronidase, generating the yellow-colored, green-fluorescent fluorescein (Abs/Em of the reaction product: 495/518 nm).
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5-Bromo-4-chloro-1H-indol-3-yl nonanoate
0 ImagesCat. No.: HY-W283888CAS No.: 133950-77-35-Bromo-4-chloro-1H-indol-3-yl nonanoate is a fluorescent substrate used to detect enzymes. The substrate reacts with various enzymes to produce a fluorescent product, which has been shown to be active against β-galactosidase, α-chymotrypsin, and β-glucuronidase.
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β-Glucuronidase-IN-3
0 ImagesCat. No.: HY-173397CAS No.: 3081681-34-4β-Glucuronidase-IN-3 (Compound 49) is a covalent allosteric β-glucuronidase inhibitor. β-Glucuronidase-IN-3 has potent inhibitory activity against Escherichia coli β-glucuronidase (EcGUS) (IC50: 12.9 nM). β-Glucuronidase-IN-3 exerts its inhibitory effect by reversibly covalently modifying the cysteine residues (Cys28, Cys443, and Cys197) of EcGUS. β-Glucuronidase-IN-3 can be used in the study of intestinal microbiota-related diseases, especially to alleviate the toxic side effects of Irinotecan (HY-16562) and nonsteroidal anti-inflammatory drugs (NSAIDs).
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Morfamquat dichloride
0 ImagesMorfamquat dichloride (PP 745) is a bipyridine herbicide. Acute poisoning by Morfamquat dichloride strongly stimulates the reticuloendothelial system, causes lysosomal membrane damage and enzyme leakage, and interferes with the function of hepatocyte Golgi apparatus and related glucose metabolic pathways. Morfamquat dichloride significantly increases the activities of acid phosphatase and β-glucuronidase, exerting toxic effects on mice, while pre-administration of vitamin E alleviates such toxicity.
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Resorufin β-D-glucuronide sodium
0 ImagesCat. No.: HY-137783CAS No.: 125440-91-7Resorufin β-D-glucuronide sodium can be used as a substrate for β-glucuronidase.
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4'',5''-Dehydroisopsoralidin
0 ImagesCat. No.: HY-N9975CAS No.: 65639-51-24'',5''-Dehydroisopsoralidin is a β-glucuronidase inhibitor (IC50: 6.3 μM). 4'',5''-Dehydroisopsoralidin has anti-inflammatory and anti-oxidative effects. 4'',5''-Dehydroisopsoralidin can be used in the research of inflammation and cancners.
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4-Methylumbelliferyl β-D-glucuronide dihydrate
0 ImagesCat. No.: HY-W7502204-Methylumbelliferyl β-D-glucuronide dihydrate is a fluorescent substrate and fluorescent probe used to detect the activity of β-D-glucuronidase. 4-Methylumbelliferyl β-D-glucuronide dihydrate also serves as a surrogate for assessing fecal coliform contamination in aquatic environments. 4-Methylumbelliferyl β-D-glucuronide dihydrate releases 4-methylumbelliferone via enzyme-mediated cleavage of the β-glycosidic bond; the latter emits fluorescence under alkaline conditions, with an excitation wavelength of 365-366 nm and an emission wavelength of 444 nm.
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m-Tolyl-β-D-glucopyranosiduronic acid
0 ImagesCat. No.: HY-N19914CAS No.: 36328-00-4m-Tolyl-β-D-glucopyranosiduronic acid (m-Cresylglucuronide) is the glucuronide conjugate metabolite of m-Cresol (HY-Y0968), which is biosynthesized and isolated from rat urine. m-Tolyl-β-D-glucopyranosiduronic acid can be hydrolyzed by β-glucuronidase to release m-Cresol.
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Dipropyl phthalate (Standard)
0 ImagesDipropyl phthalate (Standard) is the analytical standard of Dipropyl phthalate (HY-B1944). This product is intended for research and analytical applications. Dipropyl phthalate is an immunomodulator. Dipropyl phthalate alters the activities of acid phosphatase, phenoloxidase and superoxide dismutase, increases the activity of β-glucuronidase in a dose-dependent manner, and elevates the mRNA level of hemocyanin in shrimp. Dipropyl phthalate activates the FoxO signaling pathway, interferes with cell proliferation and apoptosis, downregulates chondrocyte-related genes, and induces craniofacial cartilage developmental malformations in zebrafish embryos. Dipropyl phthalate can be used in studies related to Aeromonas veronii infection and micrognathia syndrome.
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β-Glucuronidase-IN-4
0 ImagesCat. No.: HY-162884CAS No.: 2208619-76-3EcGUS-IN-1 (Compound E-9) is a non-competitive β-glucuronidase inhibitor, with an IC50 of 2.68 μM and a Ki value of 1.64 μM. EcGUS-IN-1 can improve gastrointestinal adverse events (GIAE) caused by Escherichia coli infection by inhibiting E. coli β-glucuronidase activity.
