423762-14-5
Chemical Structure
C12FDGlcU
- CAS No.: 423762-14-5
- Formula:C44H51NO18
- Molecular Weight:881.87
IUPAC Name: (2S,2'S,3S,3'S,4S,4'S,5R,5'R,6S,6'S)-6,6'-((5-dodecanamido-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(3,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylic acid)
InChIKey: BCUYISQZSHBHEJ-ASDHSWNRSA-N
SMILES: O=C1OC2(C3=CC=C(NC(CCCCCCCCCCC)=O)C=C31)C4=CC=C(O[C@@H]5O[C@@H]([C@H]([C@@H]([C@H]5O)O)O)C(O)=O)C=C4OC6=CC(O[C@@H]7O[C@@H]([C@H]([C@@H]([C@H]7O)O)O)C(O)=O)=CC=C62
Biological Activity: C12FDGlcU is a lipophilic analog of fluorescein di-β-D-glucuronic acid. C12FDGlcU can be useful for the detection of β-glucuronidase (GUS) gene expression. C12FDGlcU can enter the cells and then be cleaved by β-glucuronidase, generating the yellow-colored, green-fluorescent fluorescein (Abs/Em of the reaction product: 495/518 nm)[1][2].
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C12FDGlcU | C12FDGlcU is a lipophilic analog of fluorescein di-β-D-glucuronic acid. C12FDGlcU can be useful for the detection of β-glucuronidase (GUS) gene expression. C12FDGlcU can enter the cells and then be cleaved by β-glucuronidase, generating the yellow-colored, green-fluorescent fluorescein (Abs/Em of the reaction product: 495/518 nm). | |||||||||||||||||||||
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References
- [1]. A simple and general method for transferring genes into plants. Science. 1985 Mar 8;227(4691):1229-31. [Content Brief]
- [2]. A J Fleming, et al. Fluorescent imaging of GUS activity and RT-PCR analysis of gene expression in the shoot apical meristem. Plant J. 1996 Oct;10(4):745-54. [Content Brief]
