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  2. 4-Methylumbelliferyl-β-D-glucuronide hydrate

4-Methylumbelliferyl-β-D-glucuronide hydrate (Synonyms: MUG)

Cat. No.: HY-D0935A Purity: 99.98%
Handling Instructions

4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm , λem=445 nm).

For research use only. We do not sell to patients.

4-Methylumbelliferyl-β-D-glucuronide hydrate Chemical Structure

4-Methylumbelliferyl-β-D-glucuronide hydrate Chemical Structure

CAS No. : 881005-91-0

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25 mg USD 72 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm , λem=445 nm).

In Vitro

4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat with λex=362 nm and λem=445 nm. The half-saturation constants, Km, that are calculated for sewage samples are close to the Km values calculated based on experimental plots presented previously for freshwaters[1].

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 150 mg/mL (405.07 mM; Need ultrasonic)

H2O : 5.2 mg/mL (14.04 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7004 mL 13.5022 mL 27.0044 mL
5 mM 0.5401 mL 2.7004 mL 5.4009 mL
10 mM 0.2700 mL 1.3502 mL 2.7004 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[1]

GLUase activity of E. coli cells is measured. Three millilitres of 4-Methylumbelliferyl-β-D-glucuronide hydrate (MUGlu) solution (55 mg of hydrated MUGlu and 20 mL of Triton X-100 in 50 mL of sterile water) is added to each flask (final concentration:165 mg/L). The incubation temperature is 44°C. One hundred microlitres of 2 M NaOH solution is added to each 2.9-mL aliquot to obtain a pH>10 before the fluorescence measurement[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

370.31

Formula

C₁₆H₁₈O₁₀

CAS No.

881005-91-0

SMILES

O[[email protected]]([[email protected]]([[email protected]@H]([[email protected]@H](C(O)=O)O1)O)O)[[email protected]@H]1OC2=CC=C(C(O3)=C2)C(C)=CC3=O.O

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
4-Methylumbelliferyl-β-D-glucuronide hydrate
Cat. No.:
HY-D0935A
Quantity:

4-Methylumbelliferyl-β-D-glucuronide hydrate

Cat. No.: HY-D0935A