Fluorescein Di-β-D-Glucuronide

Name: Fluorescein Di-β-D-Glucuronide
Brand: MedChemExpress
SKU: HY-D1460
Rating: 4.5 (1 reviews)

Cat. No.: HY-D1460 Purity: 96.58%: Data Sheet
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Fluorescein Di-β-D-Glucuronide is an orally active fluorescent probe substrate and an indicator of β-glucuronidase (βG) activity in intestinal bacteria. Fluorescein Di-β-D-Glucuronide can be used for non-invasive time-lapse optical imaging of βG activity in intestinal bacteria in nude mice. Fluorescein Di-β-D-Glucuronide supports the quantitative analysis of bacterial βG enzyme activity and inhibition.

For research use only. We do not sell to patients.

Fluorescein Di-β-D-Glucuronide Chemical Structure

CAS No. : 134869-04-8

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Fluorescein Di-β-D-Glucuronide is a sensitive fluorescent probe used to detect bacterial Beta-glucuronidase activity in E. coli BL21 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Fluorescein Di-β-D-Glucuronide (7.3 μmol/kg; p.o.; single administration) produces a maximum total abdominal fluorescence flux of 9.4 × 10⁹ photons/second in 7-week-old Balb/c nude mice at 3 hours post-gavage. Its signal intensity in the large intestine is 9.4-fold higher than that in the fluorescein-treated control group, and it causes dose-dependent reductions in abdominal and large intestinal fluorescence signals in 7-week-old Balb/c nude mice pretreated with the Beta-glucuronidase (HY-P2803) inhibitor^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Balb/c nude (7-week-old)^[1]
Dosage:	7.3 μmol/kg
Administration:	p.o.; single dose
Result:	Detected a significant abdominal fluorescent signal, reaching a maximum total flux of 9.4 × 10⁹ photons/second at 3 hours post-gavage, which was 13.3-fold higher than that in fluorescein-treated mice. Showed a fluorescent signal in the large intestines that was 9.4-fold stronger than that in fluorescein-treated mice. Detected slight, non-significant signals in the liver, stomach, kidney, and small intestines, which were at least 50-fold weaker than the large intestine signal. Reduced the abdominal fluorescent signal. Reduced the fluorescent signal in excised large intestine.

Molecular Weight

684.55

Formula

C₃₂H₂₈O₁₇

CAS No.

134869-04-8

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1OC2(C3=CC=C(O[C@@H]4O[C@@H]([C@H]([C@@H]([C@H]4O)O)O)C(O)=O)C=C3OC5=CC(O[C@@H]6O[C@@H]([C@H]([C@@H]([C@H]6O)O)O)C(O)=O)=CC=C52)C7=CC=CC=C71

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (146.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4608 mL	7.3041 mL	14.6081 mL
5 mM	0.2922 mL	1.4608 mL	2.9216 mL
10 mM	0.1461 mL	0.7304 mL	1.4608 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 96.58%

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Data Sheet (275 KB) SDS (252 KB)

COA (252 KB) HNMR (152 KB) RP-HPLC (165 KB) LCMS (107 KB)

Handling Instructions (2659 KB)

References

[1]. Chen M, et al. Real-time imaging of intestinal bacterial β-glucuronidase activity by hydrolysis of a fluorescent probe. Sci Rep. 2017;7(1):3142. Published 2017 Jun 9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4608 mL	7.3041 mL	14.6081 mL	36.5203 mL
	5 mM	0.2922 mL	1.4608 mL	2.9216 mL	7.3041 mL
	10 mM	0.1461 mL	0.7304 mL	1.4608 mL	3.6520 mL
	15 mM	0.0974 mL	0.4869 mL	0.9739 mL	2.4347 mL
	20 mM	0.0730 mL	0.3652 mL	0.7304 mL	1.8260 mL
	25 mM	0.0584 mL	0.2922 mL	0.5843 mL	1.4608 mL
	30 mM	0.0487 mL	0.2435 mL	0.4869 mL	1.2173 mL
	40 mM	0.0365 mL	0.1826 mL	0.3652 mL	0.9130 mL
	50 mM	0.0292 mL	0.1461 mL	0.2922 mL	0.7304 mL
	60 mM	0.0243 mL	0.1217 mL	0.2435 mL	0.6087 mL
	80 mM	0.0183 mL	0.0913 mL	0.1826 mL	0.4565 mL
	100 mM	0.0146 mL	0.0730 mL	0.1461 mL	0.3652 mL