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Resveratrol (Synonyms: trans-Resveratrol)

Cat. No.: HY-16561 Purity: 98.90%
Handling Instructions

Resveratrol is one of the numerous polyphenolic compounds found in several vegetal sources, has a wide spectrum of targets with IC50 of 0.8, 1, 3.3 and 5 μM for adenylyl cyclase, IKKβ, DNA polymerase α and δ, respectively.

For research use only. We do not sell to patients.

Resveratrol Chemical Structure

Resveratrol Chemical Structure

CAS No. : 501-36-0

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
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Estimated Time of Arrival: December 31
200 mg USD 60 In-stock
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Estimated Time of Arrival: December 31
500 mg USD 96 In-stock
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    Resveratrol purchased from MCE. Usage Cited in: Chinese Journal of Cell Biology. 2015, 37(11):1522-1527.

    Effect of Resveratrol on SIRT1 expression in HK-2 cells.

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    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Resveratrol is one of the numerous polyphenolic compounds found in several vegetal sources, has a wide spectrum of targets with IC50 of 0.8, 1, 3.3 and 5 μM for adenylyl cyclase, IKKβ, DNA polymerase α and δ, respectively.

    IC50 & Target[1]

    IKKβ

    1 μM (IC50)

    Autophagy

     

    In Vitro

    Resveratrol is one of the numerous polyphenolic compounds found in several vegetal sources In the vast majority of cases, Resveratrol displays inhibitory/activatory effects in the micromolar range, which is potentially attainable pharmacologically, although targets with affinities in the nanomolar range have also been reported. Resveratrol also is a sirtuin activator[1]. MCF-7 cells are plated in DME-F12 medium supplemented with 5% FBS in the presence of increasing concentrations of Resveratrol. Control cells are treated with the same volume of vehicle only (0.1% ethanol). Resveratrol inhibits the growth of MCF-7 cells in a dose-dependent fashion. Addition of 10 μM Resveratrol results in an 82% inhibition of MCF-7 cell growth after 6 days while at 1 μM, only a 10% inhibition is observed. The cells treated with 10 μM Resveratrol have a doubling time of 60 hr whereas control cells doubled every 30 hr. Trypan blue exclusion assay shows that at concentrations of 10 μM or lower, Resveratrol does not affect cell viability (90% viable cells) whereas at 100 μM, only 50% of the cells are viable after 6 days of Resveratrol treatment. Moreover, MCF-7 cells do not undergo apoptosis after incubation with Resveratrol at concentration of 10 μM as determined by ApoAlert Annexin V Apoptosis kit[2].

    In Vivo

    The average tumor volume is reduced by treatment with Resveratrol at a dose of 50 mg/kg body weight (195.5±124.8 mm3; P<0.05) or 100 mg/kg body weight (81.7±70.5 mm3; P<0.001) compare with the vehicle-treated animals (315±94 mm3). There is a good correlation between the tumor volume and the tumor mass[3].

    Clinical Trial
    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 4.3814 mL 21.9068 mL 43.8135 mL
    5 mM 0.8763 mL 4.3814 mL 8.7627 mL
    10 mM 0.4381 mL 2.1907 mL 4.3814 mL
    Please refer to the solubility information to select the appropriate solvent.
    Cell Assay
    [2]

    Resveratrol is prepared in 0.1% ethanol[2].

    To determine the effect of Resveratrol on cell growth, MCF-7 cells are plated in 6-well plates at 105 cells per well in 2 mL of DME-F12 medium supplemented with 5% FBS in the presence or absence of increasing concentrations of Resveratrol. The cell number is measured every 2 days till day 6 with a hemocytometer after detaching the cells with trypsin-EDTA[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3]

    Resveratrol is dissolved in 5% ethanol and 25% polyethyleneglycol 400 in distilled water (Mice)[3].

    Mice[3]
    Female BALB/c (nu/nu) mice, 6 weeks old, are used. PA-1 cells (1×107 in 200 μL PBS) are injected s.c. on the right hind flank. Tumor volume (length×width×depth×0.52) is measured three times a week. After 10 days of implantation, two groups (n=10) are given Resveratrol (dissolved in 5% ethanol and 25% polyethyleneglycol 400 in distilled water) i.p. at a daily dose of 50 or 100 mg/kg body weight for consecutive 4 weeks, whereas the other group receive the vehicle only. Body weights are recorded everyday. Animals are given bromodeoxyuridine (BrdUrd; 10 mg/kg body weight, i.p.) 2 h before sacrifice. Xenograft tumors are weighed and frozen in liquid nitrogen or fixed in 10% formalin and embedded in paraffin. The BrdUrd-labeled cells in paraffin-embedded tissues are detected employing a monoclonal anti-BrdUrd antibody. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    228.24

    Formula

    C₁₄H₁₂O₃

    CAS No.

    501-36-0

    SMILES

    OC1=CC=C(/C=C/C2=CC(O)=CC(O)=C2)C=C1

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: 48 mg/mL (Need ultrasonic)

    Resveratrol is prepared in saline[4].

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    References

    Purity: 98.90%

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    Product Name:
    Resveratrol
    Cat. No.:
    HY-16561
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