2. Academic Validation
  3. Laminins in tumor-derived exosomes upregulated by ETS1 reprogram omental macrophages to promote omental metastasis of ovarian cancer

Laminins in tumor-derived exosomes upregulated by ETS1 reprogram omental macrophages to promote omental metastasis of ovarian cancer

  • Cell Death Dis. 2022 Dec 7;13(12):1028. doi: 10.1038/s41419-022-05472-7.
Haiyang Li 1,  Cheng Zeng 2,  Chang Shu 3,  Yuanyuan Cao 2,  Wengui Shao 3,  Mengjie Zhang 3,  Hongyong Cao 4,  Shuli Zhao 5 6
Affiliations

Affiliations

  • 1 Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
  • 2 General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
  • 3 General Clinical Research Center, Nanjing First Hospital, China Pharmaceutical University, Nanjing, Jiangsu, China.
  • 4 Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China. [email protected].
  • 5 General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China. [email protected].
  • 6 General Clinical Research Center, Nanjing First Hospital, China Pharmaceutical University, Nanjing, Jiangsu, China. [email protected].
PMID: 36477408 DOI: 10.1038/s41419-022-05472-7
Abstract

Tumor-derived exosomes participate in omental metastatic colonization of ovarian Cancer by inducing an adaptive response in the tumor microenvironment. However, cell-cell communication via exosomes between primary tumor cells and the microenvironment of distant omentum and the mechanism of pre-metastatic niche formation are poorly understood. Here, we demonstrated that ETS1-overexpressing ovarian Cancer cells secreted larger exosomes with higher laminin levels. In addition, ovarian Cancer exosomes could be taken up by omental macrophages through Integrin and laminin interaction. Compared with control exosomes, exosomes derived from ETS1-overexpressing ovarian Cancer cells (LV-ETS1 Exos) stimulated the polarization of more macrophages toward the M2 phenotype (CD163 marker), as well as the production of more CXCL5 and CCL2 in macrophages, via Integrin αvβ5/Akt/Sp1 signaling. In vivo experiments showed that LV-ETS1 Exos promoted omental metastasis of ovarian Cancer by mediating the tumor-promoting effect of macrophages, which could be neutralized by Integrin ανβ5 inhibitor cilengitide. These results indicated that ETS1 could drive ovarian Cancer cells to release exosomes with higher laminin levels, thereby accelerating the exosome-mediated pro-metastatic effects of omental macrophages via the Integrin αvβ5/Akt/Sp1 signaling pathway, and the Integrin ανβ5 inhibitor cilengitide could inhibit omental metastasis of ovarian Cancer driven by tumor-derived exosomes.

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