1. Dye Reagents
  2. FITC

FITC (Synonyms: Fluorescein 5-isothiocyanate)

Cat. No.: HY-66019 Purity: >98.0%
Handling Instructions

FITC is a derivative of fluorescein for the labeling of amines.

For research use only. We do not sell to patients.

FITC Chemical Structure

FITC Chemical Structure

CAS No. : 3326-32-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
100 mg USD 60 In-stock
Estimated Time of Arrival: December 31
500 mg USD 108 In-stock
Estimated Time of Arrival: December 31
1 g   Get quote  
5 g   Get quote  

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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products
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FITC is a derivative of fluorescein for the labeling of amines.

In Vitro

Annexin V-FITC/PI is employed to detect chondrocyte apoptosis[1]. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites[2].

Molecular Weight









Room temperature in continental US; may vary elsewhere.


-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (321.02 mM; Need ultrasonic)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5682 mL 12.8409 mL 25.6819 mL
5 mM 0.5136 mL 2.5682 mL 5.1364 mL
10 mM 0.2568 mL 1.2841 mL 2.5682 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.34 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.34 mM); Clear solution

*All of the co-solvents are provided by MCE.
Cell Assay

The frequency of chondrocyte apoptosis is measured by flow cytometry with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide. A total of 1×104 treated chondrocytes are collected from each group, washed in cold PBS and incubated with Annexin V-FITC and PI at room temperature for 15 min in the dark on ice. These samples are then analyzed using a fluorescence-activated cell sorter. Cell Quest software is used to analyze the percentage of apoptosis. All tests are repeated in triplicate.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.


Purity: >98.0%

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2


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