1. Dye Reagents
  2. FITC

FITC (Synonyms: Fluorescein 5-isothiocyanate)

Cat. No.: HY-66019 Purity: >98.0%
Handling Instructions

FITC is a derivative of fluorescein for the labeling of amines.

For research use only. We do not sell to patients.

FITC Chemical Structure

FITC Chemical Structure

CAS No. : 3326-32-7

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
100 mg USD 60 In-stock
Estimated Time of Arrival: December 31
500 mg USD 108 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

FITC is a derivative of fluorescein for the labeling of amines.

In Vitro

Annexin V-FITC/PI is employed to detect chondrocyte apoptosis[1]. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites[2].

Molecular Weight

389.38

Formula

C₂₁H₁₁NO₅S

CAS No.

3326-32-7

SMILES

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(N=C=S)C=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 30 mg/mL (77.05 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5682 mL 12.8409 mL 25.6819 mL
5 mM 0.5136 mL 2.5682 mL 5.1364 mL
10 mM 0.2568 mL 1.2841 mL 2.5682 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

The frequency of chondrocyte apoptosis is measured by flow cytometry with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide. A total of 1×104 treated chondrocytes are collected from each group, washed in cold PBS and incubated with Annexin V-FITC and PI at room temperature for 15 min in the dark on ice. These samples are then analyzed using a fluorescence-activated cell sorter. Cell Quest software is used to analyze the percentage of apoptosis. All tests are repeated in triplicate.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: >98.0%

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Keywords:

FITCFluorescein 5-isothiocyanateOthersInhibitorinhibitorinhibit

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Product name:
FITC
Cat. No.:
HY-66019
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