2. Fluorescent Dye
  3. Labeling Chemistry
  4. Functional Group Type
  5. Fluorescein Dyes

Fluorescein Dyes

Fluorescein Dyes (172):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-66019
    FITC 3326-32-7 98.60%
    FITC (Fluorescein Isothiocyanate), is one of the green fluorescein derivatives widely used in biology. FITC has the characteristics of high absorptivity and excellent fluorescence quantum yield. The isothiocyanate group of FITC can be combined with amino, sulfhydryl, imidazole, tyrosyl, carbonyl and other groups on the protein, so as to achieve protein labeling including antibodies and lectins. In addition to its use as a protein marker, FITC can also be used as a fluorescent protein tracer to rapidly identify pathogens by labeling antibodies, or for microsequencing of proteins and peptides (HPLC). The maximum excitation wavelength of FITC is 494 nm. Once excited, it fluoresces yellow-green at a maximum emission wavelength of 520 nm. In addition, FITC is also a hapten that can induce contact hypersensitivity (CHS) and induce an atopic dermatitis model.
    FITC
  • HY-D0208
    Fluorescein sodium 518-47-8
    Fluorescein (Uranine) sodium is widely used as a fluorescent tracer in medicinal and biological applications and tumor infected tissues tracer. Fluorescein (Uranine) sodium is a representative green fluorophore that has been widely used as a scaffold of practically useful green fluorescent probes.
    Fluorescein sodium
  • HY-D0098
    Fluorescein-5-maleimide 75350-46-8 99.48%
    Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent dye. Fluorescein-5-maleimide can be used to detect the redox state of thiols in eukaryotic cells. Fluorescein-5-maleimide can label peptides and is used to detect negatively charged nanoparticles. Fluorescein-5-maleimide can also label actin to explore its interaction with cardiac myosin-binding protein C (cMyBP-C), which helps in developing small molecule modulators for heart failure. Fluorescein-5-maleimide can screen mutant proteins that contain cysteine residues. The excitation wavelength of Fluorescein-5-maleimide is 494 nm, and the emission wavelength is 519 nm.
    Fluorescein-5-maleimide
  • HY-D0251
    Fluorescein 2321-07-5
    Fluorescein (Uranine) is widely used as a fluorescent tracer in medicinal and biological applications and tumor infected tissues tracer. Fluorescein (Uranine) is a representative green fluorophore that has been widely used as a scaffold of practically useful green fluorescent probes.
    Fluorescein
  • HY-66022
    5-FAM 76823-03-5 99.58%
    5-FAM (5-Carboxyfluorescein) is a green fluorescent reagent used for in situ labeling peptides, proteins and nucleotides. 5-FAM is a single isomer with Ex/Em of 490 nm/520 nm.
    5-FAM
  • HY-DY1088
    Fluorescein-5-maleimide (solution) 75350-46-8
    Fluorescein-5-maleimide (solution) (N-(5-Fluoresceinyl)maleimide (solution)) is a fluorescent dye. Fluorescein-5-maleimide can be used to detect the redox state of thiols in eukaryotic cells. Fluorescein-5-maleimide can label peptides and is used to detect negatively charged nanoparticles. Fluorescein-5-maleimide can also label actin to explore its interaction with cardiac myosin-binding protein C (cMyBP-C), which helps in developing small molecule modulators for heart failure. Fluorescein-5-maleimide can screen mutant proteins that contain cysteine residues. The excitation wavelength of Fluorescein-5-maleimide is 494 nm, and the emission wavelength is 519 nm.

    Solvent and concentration: DMSO: 10 mM
    Fluorescein-5-maleimide (solution)
  • HY-112624F4
    CY7-Dextran (MW 150kDa)
    CY7-Dextran (MW 150kDa)
  • HY-D2817A
    FITC-PLL8000
    FITC-PLL8000
  • HY-128868J
    FITC-Dextran (MW 1000)
    FITC-Dextran (MW 1000) is a compound that belongs to the class of fluorescent dyes. It is often used as a tracer molecule in biomedical research to label and track cells or other biological substances. FITC-Dextran consists of fluorescein isothiocyanate (FITC) and dextran, a complex carbohydrate extracted from starch. The combination of the two creates a stable fluorescent tracer that can be observed under a microscope or quantified using specialized detection instruments.
    FITC-Dextran (MW 1000)
  • HY-15941
    5(6)-FITC 27072-45-3
    5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of a fluorescent dye, characterized by high absorbance and excellent fluorescence quantum yield. The isothiocyanate group of FITC can react with various functional groups on proteins, including amines, thiols, imidazoles, tyrosines and carbonyls, enabling the labeling of proteins such as antibodies and lectins. 5(6)-FITC has a wide range of applications, including flow cytometry, immunofluorescence, protease assays and conjugation. The maximum excitation/emission wavelengths are 492/518 nm.
    5(6)-FITC
  • HY-D2348A
    ACE TFA 99.47%
    ACE TFA is a low-background, photostable fluorophore with nanomolar binding affinity for the Okra RNA aptamer. ACE TFA enables clear visualization of mRNA in live bacteria and mammalian cells, including tracking the trafficking of mRNA to stress granules and dual-color super-resolution imaging of RNA in live cells. (Ex=488 nm, Em=555 nm).
    ACE TFA
  • HY-15938
    5-FAM SE 92557-80-7
    5-FAM SE is a single isomer, is a fluorescent labeling reagent used for labeling peptides, proteins and nucleotides. 5-FAM SE can react with amines and can yield stable amine conjugates.
    5-FAM SE
  • HY-D0047
    5-CFDA 79955-27-4 98.22%
    5-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes.
    5-CFDA
  • HY-D1082
    5-DTAF 51306-35-5
    5-DTAF is a fluorescein dye that directly react with polysaccharides and other alcohols in aqueous solution at pH ＞9. The maximum of the 5-DTAF emission profile is at a wavelength of 518 nm after excitation at 488 nm. 5-DTAF can be used for biomolecular labeling, protein detection, and cell imaging.
    5-DTAF
  • HY-131008
    Fluorescein tyramide 210236-90-1 ≥99.0%
    Fluorescein tyramide is a green fluorescent reagent (λabs: 494 nm; λem: 517 nm). Fluorescein tyramide is widely used for tyramide signal amplification (TSA) with a low-abundance in IHC, ICC, in situ hybridization (FISH) and flow cytometry (FCM) applications.
    Fluorescein tyramide
  • HY-D2836
    FITC-HA (MW 1000000)
    FITC-HA (MW 1000000) is hyaluronic acid (HA) (HY-B0633A) labeled with FITC (HY-66019). FITC-HA retains the ability of HA to bind to receptors (such as CD44) and form extracellular matrices, while it can be detected by fluorescence microscopy or flow cytometry for tracing the localization, binding, internalization and metabolic pathways of HA in cells, tissues or living organisms (Ex/Em ≈ 490/520 nM).
    FITC-HA (MW 1000000)
  • HY-D0099
    Fluorescein-5-thiosemicarbazide 76863-28-0
    Fluorescein-5-thiosemicarbazide is a amine containing fluorescent probe that can be used for labeling saccharides and protein carbonyl derivatives.
    Fluorescein-5-thiosemicarbazide
  • HY-155640
    5-FAM maleimide 787632-00-2 98.54%
    Maleimide-5’FAM is a fluorescein. Maleimide-5’FAM can be used for labeling non-transduced cells.
    5-FAM maleimide
  • HY-15939
    6-FAM SE 92557-81-8 98.08%
    6-FAM SE (6-carboxyfluorescein succinimidyl ester) is a fluorescent labeling reagent. 6-FAM SE is used for oligonucleotide labeling and DNA sequencing.
    6-FAM SE
  • HY-D2875
    5-FITC tyramide 1396969-23-5
    5-FITC tyramide is a green fluorescent dye composed of FITC (HY-66019) and tyramide (Ex=488 nm, Em=525 nm). 5-FITC tyramide is utilized as reporter fluorescent substrate of horseradish peroxidase (HRP)-catalyzed deposition for tyramide signal amplification (TSA). 5-FITC tyramide can be used for multiplex immunohistochemistry (mIHC).
    5-FITC tyramide