1. Dye Reagents
  2. 5(6)-FITC

5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.

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5(6)-FITC Chemical Structure

5(6)-FITC Chemical Structure

CAS No. : 27072-45-3

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 74 In-stock
Estimated Time of Arrival: December 31
100 mg USD 67 In-stock
Estimated Time of Arrival: December 31
500 mg USD 264 In-stock
Estimated Time of Arrival: December 31
1 g   Get quote  
5 g   Get quote  

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Description

5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.

Molecular Weight

389.38

Formula

C₂₁H₁₁NO₅S

CAS No.
SMILES

OC1=CC=C(C2(O3)C(C=C(N=C=S)C=C4)=C4C3=O)C(OC5=C2C=CC(O)=C5)=C1.OC6=CC=C(C7(O8)C(C=CC(N=C=S)=C9)=C9C8=O)C(OC%10=C7C=CC(O)=C%10)=C6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 156 mg/mL (400.64 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5682 mL 12.8409 mL 25.6819 mL
5 mM 0.5136 mL 2.5682 mL 5.1364 mL
10 mM 0.2568 mL 1.2841 mL 2.5682 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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Keywords:

5(6)-FITCFluorescein 5(6)-isothiocyanate Fluorescein isothiocyanate 5- and 6- isomersOthersInhibitorinhibitorinhibit

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5(6)-FITC
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HY-15941
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