27072-45-3
27072-45-3
Chemical Structure
5(6)-FITC
Synonym(s): Fluorescein 5(6)-isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers
- CAS No.: 27072-45-3
- Formula:C21H11NO5S
- Molecular Weight:389.38
IUPAC Name: 3',6'-dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one compound with 3',6'-dihydroxy-6-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one (1:1)
InChIKey: AEWCEHLYVLRLBF-UHFFFAOYSA-N
SMILES: OC1=CC=C(C2(O3)C(C=C(N=C=S)C=C4)=C4C3=O)C(OC5=C2C=CC(O)=C5)=C1.OC6=CC=C(C7(O8)C(C=CC(N=C=S)=C9)=C9C8=O)C(OC%10=C7C=CC(O)=C%10)=C6
Biological Activity: 5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of a fluorescent dye, characterized by high absorbance and excellent fluorescence quantum yield. The isothiocyanate group of FITC can react with various functional groups on proteins, including amines, thiols, imidazoles, tyrosines and carbonyls, enabling the labeling of proteins such as antibodies and lectins. 5(6)-FITC has a wide range of applications, including flow cytometry, immunofluorescence, protease assays and conjugation. The maximum excitation/emission wavelengths are 492/518 nm[1][2][3][4].
| Cat. No. | 상품명 | Purity | 제품 설명 | Pricing | |||||||||||||||||||
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5(6)-FITC | 97.59% | 5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of a fluorescent dye, characterized by high absorbance and excellent fluorescence quantum yield. The isothiocyanate group of FITC can react with various functional groups on proteins, including amines, thiols, imidazoles, tyrosines and carbonyls, enabling the labeling of proteins such as antibodies and lectins. 5(6)-FITC has a wide range of applications, including flow cytometry, immunofluorescence, protease assays and conjugation. The maximum excitation/emission wavelengths are 492/518 nm. | ||||||||||||||||||||
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Fluorescein tyramide | 95.98% | Fluorescein tyramide is a green fluorescent reagent (λabs: 494 nm; λem: 517 nm). Fluorescein tyramide is widely used for tyramide signal amplification (TSA) with a low-abundance in IHC, ICC, in situ hybridization (FISH) and flow cytometry (FCM) applications. | ||||||||||||||||||||
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Fluorescein-6-isothiocyanate | 98.03% | Fluorescein-6-isothiocyanate is a fluorescent isomeric haptenic probes with Kds of 8.74, 2.72 and 1.88 for N-Acetyl-L-Lysine, normal mouse IgG and 4-4-20, respectively. | ||||||||||||||||||||
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5(6)-Carboxyfluorescein | 99.66% | 5(6)-Carboxyfluorescein (5(6)-FAM) is an amine-reactive pH-sensitive green fluorescent probe. 5(6)-Carboxyfluorescein (5(6)-FAM) can be used to label proteins, peptides and nucleotides. 5(6)-Carboxyfluorescein can be used for the detection of tumour areas in vivo. | ||||||||||||||||||||
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5(6)-TAMRA | 98.51% | 5(6)-TAMRA (5(6)-Carboxytetramethylrhodamine) is a fluorescent dye molecule widely used as a label for peptides and proteins (Ex/Em = 520/600 nm). | ||||||||||||||||||||
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5(6)-Aminofluorescein | 98.55% | 5(6)-Aminofluorescein (5(6)-AFM) is an amino-containing fluorescein dye primarily used for biomolecular labeling, fluorescence imaging, and as a precursor for the synthesis of fluorescent probes (Ex/Em = 495/515 nm). | ||||||||||||||||||||
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5(6)-Carboxy-2',7'-dichlorofluorescein | 99.03% | 5 (6)-Carboxy-2',7'-dichlorofluorescein is a fluorescent probe substrate that acts on MRP1 and MRP3/Mrp3, passively diffuses across cell membranes, and is actively effluxed by MRP1 in MRP1-positive cells. 5 (6)-Carboxy-2',7'-dichlorofluorescein is transported across membranes in an ATP-dependent manner, but does not stimulate ATPase activity. | ||||||||||||||||||||
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5(6)-CFDA | 98.75% | 5(6)-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes. | ||||||||||||||||||||
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5(6)-Carboxyfluorescein diisobutyrate | 5(6)-Carboxyfluorescein diisobutyrate is a protected 5(6)-carboxyfluorescein. 5(6)-carboxyfluorescein is a fluorescence pH indicator. | |||||||||||||||||||||
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5-FITC tyramide | 96.81% | 5-FITC tyramide is a green fluorescent dye composed of FITC (HY-66019) and tyramide (Ex=488 nm, Em=525 nm). 5-FITC tyramide is utilized as reporter fluorescent substrate of horseradish peroxidase (HRP)-catalyzed deposition for tyramide signal amplification (TSA). 5-FITC tyramide can be used for multiplex immunohistochemistry (mIHC). | ||||||||||||||||||||
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FITC (solution) | FITC (Fluorescein Isothiocyanate) (solution) , is one of the green fluorescein derivatives widely used in biology. FITC has the characteristics of high absorptivity and excellent fluorescence quantum yield. The isothiocyanate group of FITC can be combined with amino, sulfhydryl, imidazole, tyrosyl, carbonyl and other groups on the protein, so as to achieve protein labeling including antibodies and lectins. In addition to its use as a protein marker, FITC can also be used as a fluorescent protein tracer to rapidly identify pathogens by labeling antibodies, or for microsequencing of proteins and peptides (HPLC). The maximum excitation wavelength of FITC is 494 nm. Once excited, it fluoresces yellow-green at a maximum emission wavelength of 520 nm. In addition, FITC is also a hapten that can induce contact hypersensitivity (CHS) and induce an atopic dermatitis model. Solvent and concentration: DMSO: 20 mM The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base. |
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References
- [1]. J S Miller , ET AL. Flow cytometric identification of microorganisms by dual staining with FITC and PI. Cytometry. 1990;11(6):667-75. [Content Brief]
- [2]. S S Twining, et al. Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes.Anal Biochem. 1984 Nov 15;143(1):30-4. [Content Brief]
- [3]. K Sugiura, et al. A simple quantitative immunofluorescence assay for FITC-labeled antibody on platelets. J Immunol Methods. 1981;40(2):165-70. [Content Brief]
- [4]. Natalia Oddone, et al. Cell uptake mechanisms of PAMAM G4-FITC dendrimer in human myometrial cells. J Nanopart Res. 2013 15:1776.
