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  3. 5(6)-Aminofluorescein

5(6)-Aminofluorescein  (Synonyms: 5(6)-AFM; Fluoresceinamine mixed isomers)

Cat. No.: HY-D0029 Purity: 98.09%
COA Handling Instructions

5(6)-Aminofluorescein (5(6)-AFM) is a a precursor for synthesis of 5(6)-FITC (HY-15941). 5(6)-FITC is an amine-reactive derivative of fluorescein dye.

For research use only. We do not sell to patients.

5(6)-Aminofluorescein Chemical Structure

5(6)-Aminofluorescein Chemical Structure

CAS No. : 27599-63-9

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 68 In-stock
Solution
10 mM * 1 mL in DMSO USD 68 In-stock
Solid
100 mg USD 62 In-stock
500 mg USD 83 In-stock
1 g USD 110 In-stock
5 g   Get quote  
10 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 5(6)-Aminofluorescein

  • Biological Activity

  • Purity & Documentation

  • Customer Review

Description

5(6)-Aminofluorescein (5(6)-AFM) is a a precursor for synthesis of 5(6)-FITC (HY-15941). 5(6)-FITC is an amine-reactive derivative of fluorescein dye.

Molecular Weight

347.32

Formula

C20H13NO5

CAS No.
Appearance

Solid

Color

Grayish brown to reddish brown

Emission (Em)

515

Excitation (Ex)

495

SMILES

NC(C=C1)=CC2=C1C3(OC2=O)C(C=CC(O)=C4)=C4OC5=C3C=CC(O)=C5.O=C(C6=C7C=C(N)C=C6)OC87C(C=CC(O)=C9)=C9OC%10=C8C=CC(O)=C%10

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (48.00 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8792 mL 14.3959 mL 28.7919 mL
5 mM 0.5758 mL 2.8792 mL 5.7584 mL
10 mM 0.2879 mL 1.4396 mL 2.8792 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 0.62 mg/mL (1.79 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 0.62 mg/mL (1.79 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation
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5(6)-Aminofluorescein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
5(6)-Aminofluorescein
Cat. No.:
HY-D0029
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