5(6)-Aminofluorescein
Based on 1 publication(s) in Google Scholar
5(6)-Aminofluorescein (5(6)-AFM) is an amino-containing fluorescein dye primarily used for biomolecular labeling, fluorescence imaging, and as a precursor for the synthesis of fluorescent probes (Ex/Em = 495/515 nm).
For research use only. We do not sell to patients.
- Purity: 98.55%
- CAS No.: 27599-63-9
- Formula: C20H13NO5
- Molecular Weight:347.32
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 5(6)-Aminofluorescein
More
Biological Activity
As a fluorescent precursor, 5(6)-Aminofluorescein typically needs to be converted into an active ester (such as NHS) before being used for the fluorescent labeling of proteins or nucleic acids.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
515
495
Chemical Information
-
CAS No. 27599-63-9
-
Appearance Solid
-
Molecular Weight 347.32
-
Formula C20H13NO5
-
Color Grayish brown to reddish brown
-
SMILES
NC(C=C1)=CC2=C1C3(OC2=O)C(C=CC(O)=C4)=C4OC5=C3C=CC(O)=C5.O=C(C6=C7C=C(N)C=C6)OC87C(C=CC(O)=C9)=C9OC%10=C8C=CC(O)=C%10
-
Synonyms
5(6)-AFM; Fluoresceinamine mixed isomers
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Carbohydr Polym
2021 Aug 1:265:118064. PMID: 33966828
Solvent & Solubility
DMSO : 16.67 mg/mL (48.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (248 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8792 mL | 14.3959 mL | 28.7919 mL | 71.9797 mL |
| 5 mM | 0.5758 mL | 2.8792 mL | 5.7584 mL | 14.3959 mL | |
| 10 mM | 0.2879 mL | 1.4396 mL | 2.8792 mL | 7.1980 mL | |
| 15 mM | 0.1919 mL | 0.9597 mL | 1.9195 mL | 4.7986 mL | |
| 20 mM | 0.1440 mL | 0.7198 mL | 1.4396 mL | 3.5990 mL | |
| 25 mM | 0.1152 mL | 0.5758 mL | 1.1517 mL | 2.8792 mL | |
| 30 mM | 0.0960 mL | 0.4799 mL | 0.9597 mL | 2.3993 mL | |
| 40 mM | 0.0720 mL | 0.3599 mL | 0.7198 mL | 1.7995 mL |
5(6)-Aminofluorescein Related Classifications
HY-D0029 Related Classifications
Powered by Bioz