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  4. 5(6)-Aminofluorescein

5(6)-Aminofluorescein  (Synonyms: 5(6)-AFM; Fluoresceinamine mixed isomers)

Cat. No.: HY-D0029 Purity: 98.55%
COA
SDS
Handling Instructions Technical Support

5(6)-Aminofluorescein (5(6)-AFM) is an amino-containing fluorescein dye primarily used for biomolecular labeling, fluorescence imaging, and as a precursor for the synthesis of fluorescent probes (Ex/Em = 495/515 nm).

For research use only. We do not sell to patients.

5(6)-Aminofluorescein

5(6)-Aminofluorescein Chemical Structure

CAS No. : 27599-63-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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Based on 1 publication(s) in Google Scholar

5(6)-Aminofluorescein Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 5(6)-Aminofluorescein

  • Biological Activity

  • Purity & Documentation

  • Customer Review

Description

5(6)-Aminofluorescein (5(6)-AFM) is an amino-containing fluorescein dye primarily used for biomolecular labeling, fluorescence imaging, and as a precursor for the synthesis of fluorescent probes (Ex/Em = 495/515 nm).

In Vitro

As a fluorescent precursor, 5(6)-Aminofluorescein typically needs to be converted into an active ester (such as NHS) before being used for the fluorescent labeling of proteins or nucleic acids.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5(6)-Aminofluorescein Related Antibodies
Molecular Weight

347.32

Formula

C20H13NO5
CAS No.
Appearance

Solid

Color

Grayish brown to reddish brown

Emission (Em)

515
Excitation (Ex)

495
SMILES

NC(C=C1)=CC2=C1C3(OC2=O)C(C=CC(O)=C4)=C4OC5=C3C=CC(O)=C5.O=C(C6=C7C=C(N)C=C6)OC87C(C=CC(O)=C9)=C9OC%10=C8C=CC(O)=C%10
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (48.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8792 mL 14.3959 mL 28.7919 mL
5 mM 0.5758 mL 2.8792 mL 5.7584 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.55%

SDS (393 KB)

COA (252 KB) HNMR (399 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8792 mL 14.3959 mL 28.7919 mL 71.9797 mL
5 mM 0.5758 mL 2.8792 mL 5.7584 mL 14.3959 mL
10 mM 0.2879 mL 1.4396 mL 2.8792 mL 7.1980 mL
15 mM 0.1919 mL 0.9597 mL 1.9195 mL 4.7986 mL
20 mM 0.1440 mL 0.7198 mL 1.4396 mL 3.5990 mL
25 mM 0.1152 mL 0.5758 mL 1.1517 mL 2.8792 mL
30 mM 0.0960 mL 0.4799 mL 0.9597 mL 2.3993 mL
40 mM 0.0720 mL 0.3599 mL 0.7198 mL 1.7995 mL
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5(6)-Aminofluorescein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5(6)-Aminofluorescein27599-63-95(6)-AFM Fluoresceinamine mixed isomersFluorescent DyeInhibitorinhibitorinhibit

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