Fluorescein-6-isothiocyanate

Cat. No.: HY-136957 Purity: 98.03%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Fluorescein-6-isothiocyanate is a fluorescent isomeric haptenic probes with K_ds of 8.74, 2.72 and 1.88 for N-Acetyl-L-Lysine, normal mouse IgG and 4-4-20, respectively.

For research use only. We do not sell to patients.

Fluorescein-6-isothiocyanate Chemical Structure

CAS No. : 18861-78-4

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Fluorescein-6-isothiocyanate is a fluorescent isomeric haptenic probes with K_ds of 8.74, 2.72 and 1.88 for N-Acetyl-L-Lysine, normal mouse IgG and 4-4-20, respectively^[1].

IC₅₀ & Target

K_d: 8.74 (N-Acetyl-L-Lysine), 2.72 (normal mouse IgG), 1.88 (4-4-20)^[1]

Molecular Weight

389.38

Formula

C₂₁H₁₁NO₅S

CAS No.

18861-78-4

Appearance

Solid

Color

Light yellow to brown

Emission (Em)

525

Excitation (Ex)

488

SMILES

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=CC(N=C=S)=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (642.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5682 mL	12.8409 mL	25.6819 mL
5 mM	0.5136 mL	2.5682 mL	5.1364 mL
10 mM	0.2568 mL	1.2841 mL	2.5682 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.03%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (257 KB) HNMR (263 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Swindlehurst CA, et al. Fluorescence measurements of immune complexes of Mab 4-4-20 with isomeric haptens. Biophys J. 1991 Mar;59(3):619-28. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5682 mL	12.8409 mL	25.6819 mL	64.2046 mL
	5 mM	0.5136 mL	2.5682 mL	5.1364 mL	12.8409 mL
	10 mM	0.2568 mL	1.2841 mL	2.5682 mL	6.4205 mL
	15 mM	0.1712 mL	0.8561 mL	1.7121 mL	4.2803 mL
	20 mM	0.1284 mL	0.6420 mL	1.2841 mL	3.2102 mL
	25 mM	0.1027 mL	0.5136 mL	1.0273 mL	2.5682 mL
	30 mM	0.0856 mL	0.4280 mL	0.8561 mL	2.1402 mL
	40 mM	0.0642 mL	0.3210 mL	0.6420 mL	1.6051 mL
	50 mM	0.0514 mL	0.2568 mL	0.5136 mL	1.2841 mL
	60 mM	0.0428 mL	0.2140 mL	0.4280 mL	1.0701 mL
	80 mM	0.0321 mL	0.1605 mL	0.3210 mL	0.8026 mL
	100 mM	0.0257 mL	0.1284 mL	0.2568 mL	0.6420 mL