5-FITC tyramide
Based on 1 publication(s) in Google Scholar
5-FITC tyramide is a green fluorescent dye composed of FITC (HY-66019) and tyramide (Ex=488 nm, Em=525 nm). 5-FITC tyramide is utilized as reporter fluorescent substrate of horseradish peroxidase (HRP)-catalyzed deposition for tyramide signal amplification (TSA). 5-FITC tyramide can be used for multiplex immunohistochemistry (mIHC).
For research use only. We do not sell to patients.
- Purity: 96.81%
- CAS No.: 1396969-23-5
- Formula: C29H22N2O6S
- Molecular Weight:526.56
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 5-FITC tyramide
More
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
5-FITC tyramide assay
1. Inject into the rat ganglion with 4 μL of WGA-HRP tracer, and then get specific tissues for slide.
2. Slides were allowed to dry for 60 min.
3. Rehydrate in TNT buffer (0.1 M Tris-HCl, 0.15 M NaCl, 0.05% Tween 20, pH 7.5), and then quench in freshly prepared NaBH4 (0.5 mg/mL) in TN buffer (0.1 M Tris-HCl, 0.15 M NaCl, pH 7.5) for 15 min.
4. Wash in TNT and then permeabilized in 0.1% Triton X-100 in TN for 15 min.
5. Incubate in TNT plus 2% BSA (TNT-BSA) for 60 min following washing in TNT, and then incubate min in 5-FITC tyramide for 15 min (1: 50 with amplification diluent).
6. Wash in TNT, and then apply the primary antibodies overnight at 4°C following detecting by incubation with donkey anti-rabbit IgG conjugated to Lissamine rhodamine (LRSC) or Cy3 (HY-D0822) (1:200 with TNT-BSA) and donkey anti-sheep Cy5 (HY-D0821) (1:200 with TNT-BSA).
7. Fluorescence microscopy detection.
Note: it is suggested that the optimal concentration of 5-FITC tyramide working solution according to actual conditions.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1396969-23-5
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Appearance Solid
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Molecular Weight 526.56
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Formula C29H22N2O6S
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Color Orange to red
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SMILES
OC1=CC=C(CCNC(NC2=CC=C(C3(OC4=O)C5=CC=C(C=C5OC6=CC(O)=CC=C63)O)C4=C2)=S)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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J Adv Res
RNA m6A-binding protein YTHDF2 regulates neoblast proliferation in planarian regeneration by targeting ASTACIN Signaling. [Abstract]2025 Dec 11:S2090-1232(25)00997-X. PMID: 41390116
Solvent & Solubility
DMSO : 50 mg/mL (94.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8991 mL | 9.4956 mL | 18.9912 mL | 47.4780 mL |
| 5 mM | 0.3798 mL | 1.8991 mL | 3.7982 mL | 9.4956 mL | |
| 10 mM | 0.1899 mL | 0.9496 mL | 1.8991 mL | 4.7478 mL | |
| 15 mM | 0.1266 mL | 0.6330 mL | 1.2661 mL | 3.1652 mL | |
| 20 mM | 0.0950 mL | 0.4748 mL | 0.9496 mL | 2.3739 mL | |
| 25 mM | 0.0760 mL | 0.3798 mL | 0.7596 mL | 1.8991 mL | |
| 30 mM | 0.0633 mL | 0.3165 mL | 0.6330 mL | 1.5826 mL | |
| 40 mM | 0.0475 mL | 0.2374 mL | 0.4748 mL | 1.1869 mL | |
| 50 mM | 0.0380 mL | 0.1899 mL | 0.3798 mL | 0.9496 mL | |
| 60 mM | 0.0317 mL | 0.1583 mL | 0.3165 mL | 0.7913 mL | |
| 80 mM | 0.0237 mL | 0.1187 mL | 0.2374 mL | 0.5935 mL |
5-FITC tyramide Related Classifications
HY-D2875 Related Classifications
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