1. Dye Reagents

Zy3 carboxylic acid 

Cat. No.: HY-D0822 Purity: >98.0%
Handling Instructions

Zy3 carboxylic acid (Compound 7c) is a novel cyanine dyes with vinylsulfone group for labeling biomolecules with maximum wavelength (λabs) and emission maximum wavelength (λem) of 549 nm and 566 nm, respectively.

For research use only. We do not sell to patients.
Zy3 carboxylic acid Chemical Structure

Zy3 carboxylic acid Chemical Structure

CAS No. : 146368-13-0

Size Price Stock Quantity
10 mM * 1 mL in Water USD 366 In-stock
5 mg USD 264 In-stock
10 mg   Get quote  
50 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Zy3 carboxylic acid (Compound 7c) is a novel cyanine dyes with vinylsulfone group for labeling biomolecules with maximum wavelength (λabs) and emission maximum wavelength (λem) of 549 nm and 566 nm, respectively.

In Vitro

To ensure labeling efficiency, whether there is any effect from the difference in the length of the spacer is determine. For the 550 nm range, 7a, Zy3 carboxylic acid (Compound 7c), 7f, and 7g having five, six, eight, and eight carbon atoms in the spacer, respectively, are chosen. For the spacer-dependent reactivity test, dyes with the highest extinction coefficients (7g, 7l, and 7n) and those with ethyl groups as R on indole (7a, Zy3 carboxylic acid, 7f, and 7i) are selected. As a result, dyes with longer spacers are observed to have aster binding affinity to BSA. Among 7a, Zy3 carboxylic acid, 7f, and 7g, with the same absorption wavelength at ~550 nm (m=1)[1].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.5854 mL 7.9268 mL 15.8536 mL
5 mM 0.3171 mL 1.5854 mL 3.1707 mL
10 mM 0.1585 mL 0.7927 mL 1.5854 mL
Please refer to the solubility information to select the appropriate solvent.
Kinase Assay
[1]

Stock solutions are prepared by dissolving bovine serum albumin (BSA, 10 mg/mL) in 0.1 M sodium phosphate buffer (pH 9.5) and 7a, Zy3 carboxylic acid (Compound 7c), 7f, 7g, 7i, 7l, and 7n (10 mg/mL) in distilled water. Each dye solution (7a (11.0 μL), Zy3 carboxylic acid (11.2 μL), 7f (11.6 μL), 7g (11.9 μL), 7i (11.7 μL), 7l (12.1 μL), and 7n (12.1 μL)) is reacted with the BSA solution (1 mL) in equivalent at 25 °C for 6 h. The labeling reactions are then monitored every 1 h via HPLC at 550, 650, and 750 nm (C6-Phenyl Column, 15-25% acetonitrile-water, 1.5 mL/min)[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

630.77

Formula

C₃₁H₃₈N₂O₈S₂

CAS No.

146368-13-0

SMILES

CC(/C(N1CCCCCC(O)=O)=C\C=C\C2=[N+](CC)C(C=CC(S([O-])(=O)=O)=C3)=C3C2(C)C)(C)C4=C1C=CC(S(=O)(O)=O)=C4

Storage

-20°C, protect from light

Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

H2O: 16 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
Zy3 carboxylic acid
Cat. No.:
HY-D0822
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