CY3
Based on 4 publication(s) in Google Scholar
Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554, λem=568).
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 146368-13-0
- Formula: C31H38N2O8S2
- Molecular Weight:630.77
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) CY3
More
Customer Validation & Images
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Cell Imaging/Staining
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IF
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IF
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Cell Imaging/Staining
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Cell Imaging/Staining
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Protein Preparation
To obtain the best labeling effect, please prepare the protein (antibody) concentration to 2 mg/mL.
1.1 The pH value of the protein solution should be 8.5 ± 0.5. If the pH is lower than 8.0, adjust it with 1 M sodium bicarbonate.
1.2 If the protein concentration is lower than 2 mg/mL, the labeling efficiency will be greatly reduced. To obtain the best labeling efficiency, it is recommended that the final protein concentration range be 2-10 mg/mL.
1.3 The protein must be in a buffer free of primary amines (such as Tris or glycine) and ammonium ions, otherwise the labeling efficiency will be affected.
Note: Before use, it must be activated with condensation solution (500 μg/mL) (HY-D0178) before subsequent labeling experiments can be performed.
2. Dye Preparation
Dilute the dye with anhydrous DMSO to prepare a clear stock solution of 10 mg/mL.
3. Calculation of Dye Amount
The amount of dye required for the reaction depends on the amount of protein to be labeled. The optimal molar ratio of dye to protein is approximately 10:1.
Example: Assuming the required labeled protein is 500 μL 2 mg/mL IgG (MW=150,000), and 1 mg of dye is dissolved in 100 μL DMSO, the required dye volume is 4.2 μL. The detailed calculation process is as follows:
1) mmol (IgG) = mg/mL (IgG) × mL (IgG)/MW (IgG) = 2 mg/mL × 0.5 mL/150,000 mg/mmol = 6.7 × 10-6 mmol
2) mmol (dye) = mmol (IgG) × 10 = 6.7 × 10-6 mmol × 10 = 6.7 × 10-5 mmol
3) μL (dye) = mmol (dye) × MW (dye)/mg/μL (dye) = 6.7 ×10-5 mmol × 630.77 mg/mmol/0.01 mg/μL = 4.2 μL (dye)
Note: We recommend using a dye:protein molar ratio of 10:1. If the concentration is too low or too high, the ratio can be adjusted to 5:1, 15:1, or 20:1.
4. Running the Coupling Reaction
1) Slowly add the calculated volume of freshly prepared 10 mg/mL dye to 0.5 mL of protein sample solution, gently shake to mix, and then briefly centrifuge to collect the sample at the bottom of the reaction tube. Avoid vigorous mixing to prevent denaturation and inactivation of the protein sample.
2) Place the reaction tube in a dark place and incubate gently with shaking for 60 minutes at room temperature. Every 10-15 minutes, gently invert the reaction tube several times to thoroughly mix the reactants and improve labeling efficiency.
5. Purification of Conjugates
The following protocol is an example of purifying dye-protein conjugates using a Labelling Kits Centrifugation-Based Rapid Desalting Column (5KD) (HY-D3014).
5.1 Prepare the desalting column according to the instructions.
5.2 Load the reaction mixture onto the top of the desalting column.
5.3 Once the sample has run below the resin surface, immediately add PBS (pH 7.2-7.4).
5.4 Add more PBS (pH 7.2-7.4) to the target sample to complete column purification. Collect the fraction containing the desired dye-protein conjugate.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
570
550
Chemical Information
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CAS No. 146368-13-0
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Appearance Solid
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Molecular Weight 630.77
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Formula C31H38N2O8S2
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Color Purple to purplish red
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SMILES
CC(/C(N1CCCCCC(O)=O)=C\C=C\C2=[N+](CC)C(C=CC(S([O-])(=O)=O)=C3)=C3C2(C)C)(C)C4=C1C=CC(S(=O)(O)=O)=C4
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Synonyms
Sulfo-Cyanine3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (4)
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Journal Impact Factor
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Most Recent
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ACS Nano
UiO-66 MOFs-Based "Epi-Nano-Sonosensitizer" for Ultrasound-Driven Cascade Immunotherapy against B-Cell Lymphoma. [Abstract]2025 Feb 18;19(6):6282-6298. PMID: 39920081
CY3 purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Feb 18;19(6):6282-6298. [Abstract]
Representative fluorescence images showing the uptake of Cy3-labeled CRUPPA19 (red) by A20 cells and normal monocytes (L929) after 30 min of incubation. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar: 20 μm.
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Nat Commun
2025 Apr 24;16(1):3855. PMID: 40274828
CY3 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Apr 24;16(1):3855. [Abstract]
Fluorescence images of in vitro mixtures of His-YAP-mEGFP (green) and His-MED15-CY3 (red) at the indicated module concentrations in 10% PEG solution, incubated overnight at 4°C on a shaker.
CY3 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Apr 24;16(1):3855. [Abstract]
The plot shows the mean droplet size. DIC images of the His-YAP-mEGFP and His-MED15-CY3 mixture (1:1 at 5 µM, in 10% PEG) show numerous droplets at room temperature in a coverslip chamber.
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Adv Sci (Weinh)
Functionalized Biomimetic Nanoparticles Targeting the IL-10/IL-10Rα/Glycolytic Axis in Synovial Macrophages Alleviate Cartilage Degeneration in Osteoarthritis. [Abstract]2025 May 2:e2504768. PMID: 40317692
CY3 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 2:e2504768. [Abstract]
Confocal microscopy image of macrophages phagocytosing MI@UN. Nuclei were stained with DAPI (blue), MM with DiO (green), IL-10 with CY3 (red), and NO-prednisolone with CY5.5 (magenta). Scale bar: 20 µm.
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Mater Today Bio
GGT and GSH-triggered nanoplatform for efficient gambogic acid delivery and tumor penetration in triple-negative breast cancer. [Abstract]2025 Jun 27:33:102030. PMID: 40677397
CY3 purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Jun 27:33:102030. [Abstract]
Time-dependent cellular uptake of the Cy3-labeled, GA-loaded nanoplatform (red) in 4T1 cells, visualized by CLSM. Nuclei were stained with DAPI (blue). Scale bar: 100 µm.
Solvent & Solubility
DMSO : 100 mg/mL (158.54 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 12.5 mg/mL (19.82 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 1.5854 mL | 7.9268 mL | 15.8536 mL | 39.6341 mL |
| 5 mM | 0.3171 mL | 1.5854 mL | 3.1707 mL | 7.9268 mL | |
| 10 mM | 0.1585 mL | 0.7927 mL | 1.5854 mL | 3.9634 mL | |
| 15 mM | 0.1057 mL | 0.5285 mL | 1.0569 mL | 2.6423 mL | |
| DMSO | 20 mM | 0.0793 mL | 0.3963 mL | 0.7927 mL | 1.9817 mL |
| 25 mM | 0.0634 mL | 0.3171 mL | 0.6341 mL | 1.5854 mL | |
| 30 mM | 0.0528 mL | 0.2642 mL | 0.5285 mL | 1.3211 mL | |
| 40 mM | 0.0396 mL | 0.1982 mL | 0.3963 mL | 0.9909 mL | |
| 50 mM | 0.0317 mL | 0.1585 mL | 0.3171 mL | 0.7927 mL | |
| 60 mM | 0.0264 mL | 0.1321 mL | 0.2642 mL | 0.6606 mL | |
| 80 mM | 0.0198 mL | 0.0991 mL | 0.1982 mL | 0.4954 mL | |
| 100 mM | 0.0159 mL | 0.0793 mL | 0.1585 mL | 0.3963 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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