1-Ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride
Based on 14 publication(s) in Google Scholar
1-Ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride is a carbodiimide reagent that can form nucleic acid and compounds with amide bonds. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride accelerates the formation reaction of esters, amides, and peptides, as a condensing and dehydrating agent, which are often used for polynucleotide synthesis, anhydroxydation, lactonization and esterification.
For research use only. We do not sell to patients.
- Purity : 99.80%
- CAS No.: 25952-53-8
- Formula: C8H18ClN3
- Molecular Weight:191.70
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-Y0623
N-Hydroxysuccinimide -
HY-Y1092
N,N-Dicyclohexylcarbodiimide(DCC) -
HY-D0924
Cy5.5 -
HY-W002213
N-Hydroxysulfosuccinimide sodium -
HY-Y1089
4-Dimethylaminopyridine -
HY-D0821
CY5 -
HY-Y1703
HATU -
HY-Y1092R
N,N-Dicyclohexylcarbodiimide(DCC) (Standard) -
HY-Y1168
DMTMM -
HY-79880
N,N'-Carbonyldiimidazole -
HY-Y0912
HBTU -
HY-D0858
MES
Publications Citing Use of MedChemExpress (MCE) 1-Ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride
More- J Nanobiotechnology. 2025 Aug 26;23(1):588. [Abstract]
- Adv Sci (Weinh). 2026 Jun 9:e76004. [Abstract]
- Adv Sci (Weinh). 2025 Jan;12(1):e2410769. [Abstract]
- Adv Sci (Weinh). 2024 Dec;11(48):e2408044. [Abstract]
- Adv Healthc Mater. 2026 Feb 21:e05684. [Abstract]
- View. 2026 Mar 22.
- Ecotoxicol Environ Saf. 2025 Nov 15:307:119433. [Abstract]
- Cell Biol Toxicol. 2025 May 15;41(1):85. [Abstract]
- Arab J Chem. 2025 Nov 14.
- Microchem J. 2026 Mar 2.
- IEEE Sens J. 2026.
- Biomed Mater. 2026 Apr 20;21(2). [Abstract]
- bioRxiv. 2025 Dec 5.
- Med Drug Discov. 2025 Sep 20;28:100226.
Biological Activity
Description
In Vitro
Usage:
First dilute 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to 500 μg/mL, mix with the dye, the volume ratio of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to the dye is 1:1, incubate at room temperature for about 20 minutes, then add them to the sample solution and incubate at room temperature for 1-2 hours.
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) is a very useful agent to form amide bonds (peptide bonds) in an aqueous medium[1].
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl), is widely used for polyaniline-carbon nanotube preparation for a cholesterol biosensor, precolumn derivatization of aliphatic amines for HPLC, molecular beacons formation for DNA reseach, sensor preparation for calcium detection, the fluorescent determination of carboxylic acids, and solidphase microsequencing of peptides[2].
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) is an aminoaldehyde dehydrogenase (AMADH) inhibitor, while its inhibition on glutamate decarboxylase (GAD) is insignificant[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 25952-53-8
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Appearance Solid
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Molecular Weight 191.70
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Formula C8H18ClN3
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Color White to off-white
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SMILES
CN(C)CCCN=C=NCC.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (14)
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Journal Impact Factor
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Most Recent
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J Nanobiotechnology
Targeted neural stem cell-derived extracellular vesicles loaded with Sinomenine alleviate diabetic peripheral neuropathy via WNT5a/TRPV1 pathway modulation. [Abstract]2025 Aug 26;23(1):588. PMID: 40855286 -
Adv Sci (Weinh)
A Brain-Penetrant Nanobody Reveals GSK3β-Driven Proline-Directed Phosphorylation as a Master Regulator of Ischemic Neurodegeneration. [Abstract]2026 Jun 9:e76004. PMID: 42261803 -
Adv Sci (Weinh)
Gene Therapy for Inflammatory Cascade in Intrauterine Injury with Engineered Extracellular Vesicles Hybrid Snail Mucus-enhanced Adhesive Hydrogels. [Abstract]2025 Jan;12(1):e2410769. PMID: 39454114 -
Adv Sci (Weinh)
Concrete-Inspired Bionic Bone Glue Repairs Osteoporotic Bone Defects by Gluing and Remodeling Aging Macrophages. [Abstract]2024 Dec;11(48):e2408044. PMID: 39455287 -
Adv Healthc Mater
An Injectable Liposome-Hydrogel Composite for Targeted Delivery of a Non-Lactylated Peptide to Rebalance Bone Metabolism in Hyperlipidemia. [Abstract]2026 Feb 21:e05684. PMID: 41721628 -
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Ecotoxicol Environ Saf
PPARγ-responsive luciferase reporter system for high-throughput screening of chemical toxins with potential pulmonary fibrosis effects. [Abstract]2025 Nov 15:307:119433. PMID: 41273832 -
Cell Biol Toxicol
IGFBP3 enhances adipose-derived stem cell function in soft tissue injury repair via ITGB1 and ERK pathway activation. [Abstract]2025 May 15;41(1):85. PMID: 40369223 -
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Biomed Mater
Synthesis of citric acid-coated nanomaterials releasing oxygen and antioxidant vitamin E and investigation of their effects on healthy and cancer cells under hypoxic and normoxic conditions. [Abstract]2026 Apr 20;21(2). PMID: 41962577 -
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Solvent & Solubility
In Vitro:
H2O : ≥ 100 mg/mL (521.65 mM)
DMSO : 62.5 mg/mL (326.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (10.85 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (10.85 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (731 KB)
- English - EN (731 KB)
- Français - FR (731 KB)
- Deutsch - DE (731 KB)
- Norwegian - NO (731 KB)
- Español - ES (731 KB)
- Swedish - SV (731 KB)
- Italian - IT (731 KB)
- Korean - KR (731 KB)
- Portuguese - PT (731 KB)
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Handling Instructions (2659 KB)
References
[1]. Kunihiko Seno, et al. Spectrophotometric determination of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow injection analysis. Anal Sci. 2008 Apr;24(4):505-8. [Content Brief]
[2]. Kunihiko Seno, et al. Determination of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow-injection analysis based on a specific condensation reaction between malonic acid and ethylenediamine. Anal Sci. 2009 Mar;25(3):389-93. [Content Brief]
[3]. Runqiang Yang, et al. AMADH inhibitor optimization and its effects on GABA accumulation in soybean sprouts under NaCl-CaCl 2 treatment. 3 Biotech. 2019 May;9(5):184. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 5.2165 mL | 26.0824 mL | 52.1648 mL | 130.4121 mL |
| 5 mM | 1.0433 mL | 5.2165 mL | 10.4330 mL | 26.0824 mL | |
| 10 mM | 0.5216 mL | 2.6082 mL | 5.2165 mL | 13.0412 mL | |
| 15 mM | 0.3478 mL | 1.7388 mL | 3.4777 mL | 8.6941 mL | |
| 20 mM | 0.2608 mL | 1.3041 mL | 2.6082 mL | 6.5206 mL | |
| 25 mM | 0.2087 mL | 1.0433 mL | 2.0866 mL | 5.2165 mL | |
| 30 mM | 0.1739 mL | 0.8694 mL | 1.7388 mL | 4.3471 mL | |
| 40 mM | 0.1304 mL | 0.6521 mL | 1.3041 mL | 3.2603 mL | |
| 50 mM | 0.1043 mL | 0.5216 mL | 1.0433 mL | 2.6082 mL | |
| 60 mM | 0.0869 mL | 0.4347 mL | 0.8694 mL | 2.1735 mL | |
| 80 mM | 0.0652 mL | 0.3260 mL | 0.6521 mL | 1.6302 mL | |
| 100 mM | 0.0522 mL | 0.2608 mL | 0.5216 mL | 1.3041 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
1-Ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride Related Classifications
HY-D0178 Related Classifications
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