1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride

Cat. No.: HY-D0178 Purity: 99.95%: FAQs
Data Sheet SDS COA Handling Instructions

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1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is a carbodiimide reagent that can form nucleic acid and compounds with amide bonds. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride accelerates the formation reaction of esters, amides, and peptides, as a condensing and dehydrating agent, which are often used for polynucleotide synthesis, anhydroxydation, lactonization and esterification.

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1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Chemical Structure

CAS No. : 25952-53-8

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Description

In Vitro

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) is a very useful agent to form amide bonds (peptide bonds) in an aqueous medium^[1].
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC•HCl), is widely used for polyaniline-carbon nanotube preparation for a cholesterol biosensor, precolumn derivatization of aliphatic amines for HPLC, molecular beacons formation for DNA reseach, sensor preparation for calcium detection, the fluorescent determination of carboxylic acids, and solidphase microsequencing of peptides^[2].
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC•HCl) is an aminoaldehyde dehydrogenase (AMADH) inhibitor, while its inhibition on glutamate decarboxylase (GAD) is insignificant^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

191.70

Formula

C₈H₁₈ClN₃

CAS No.

25952-53-8

Appearance

Solid

Color

White to off-white

SMILES

CN(C)CCCN=C=NCC.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (521.65 mM; Need ultrasonic)

DMSO : 62.5 mg/mL (326.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.2165 mL	26.0824 mL	52.1648 mL
5 mM	1.0433 mL	5.2165 mL	10.4330 mL
10 mM	0.5216 mL	2.6082 mL	5.2165 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (13.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (269 KB) SDS (393 KB)

COA (241 KB) HNMR (235 KB) RP-HPLC (246 KB) MS (205 KB)

Handling Instructions (2659 KB)

References

[1]. Kunihiko Seno, et al. Spectrophotometric determination of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow injection analysis. Anal Sci. 2008 Apr;24(4):505-8. [Content Brief]

[2]. Kunihiko Seno, et al. Determination of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow-injection analysis based on a specific condensation reaction between malonic acid and ethylenediamine. Anal Sci. 2009 Mar;25(3):389-93. [Content Brief]

[3]. Runqiang Yang, et al. AMADH inhibitor optimization and its effects on GABA accumulation in soybean sprouts under NaCl-CaCl 2 treatment. 3 Biotech. 2019 May;9(5):184. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	5.2165 mL	26.0824 mL	52.1648 mL	130.4121 mL
	5 mM	1.0433 mL	5.2165 mL	10.4330 mL	26.0824 mL
	10 mM	0.5216 mL	2.6082 mL	5.2165 mL	13.0412 mL
	15 mM	0.3478 mL	1.7388 mL	3.4777 mL	8.6941 mL
	20 mM	0.2608 mL	1.3041 mL	2.6082 mL	6.5206 mL
	25 mM	0.2087 mL	1.0433 mL	2.0866 mL	5.2165 mL
	30 mM	0.1739 mL	0.8694 mL	1.7388 mL	4.3471 mL
	40 mM	0.1304 mL	0.6521 mL	1.3041 mL	3.2603 mL
	50 mM	0.1043 mL	0.5216 mL	1.0433 mL	2.6082 mL
	60 mM	0.0869 mL	0.4347 mL	0.8694 mL	2.1735 mL
	80 mM	0.0652 mL	0.3260 mL	0.6521 mL	1.6302 mL
	100 mM	0.0522 mL	0.2608 mL	0.5216 mL	1.3041 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.