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  2. 5(6)-Carboxyfluorescein

5(6)-Carboxyfluorescein (Synonyms: 5(6)-FAM; 5-(and-6)-Carboxyfluorescein mixed isomers)

Cat. No.: HY-15940 Purity: 98.50%
Handling Instructions

5(6)-Carboxyfluorescein (5(6)-FAM) is an amine-reactive pH-sensitive green fluorescent probe. 5(6)-Carboxyfluorescein (5(6)-FAM) can be used to label proteins, peptides and nucleotides. 5(6)-Carboxyfluorescein can be used for the detection of tumour areas in vivo.

For research use only. We do not sell to patients.

5(6)-Carboxyfluorescein Chemical Structure

5(6)-Carboxyfluorescein Chemical Structure

CAS No. : 72088-94-9

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10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
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10 mM * 1 mL
ready for reconstitution
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Estimated Time of Arrival: December 31
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500 mg USD 50 In-stock
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Description

5(6)-Carboxyfluorescein (5(6)-FAM) is an amine-reactive pH-sensitive green fluorescent probe. 5(6)-Carboxyfluorescein (5(6)-FAM) can be used to label proteins, peptides and nucleotides. 5(6)-Carboxyfluorescein can be used for the detection of tumour areas in vivo[1][2].

In Vitro

5(6)-Carboxyfluorescein has two main characteristics: it has two wavelengths of maximum absorbance (465 and 490 nm) and its fluorescence emission (maximum, 515 nm) increases as a function of pH in the physiological pH range of 6-7.4[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

5(6)-Carboxyfluorescein (5 mg/kg; i.p.) can be used for in vivo pH mapping of tumor tissue[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: CDF mice, bearing lymphoid leukemia P388[2]
Dosage: 5 mg/kg
Administration: Injected intraperitoneally
Result: Could be used for measurement and imaging of tumor tissue.
Molecular Weight

752.63

Formula

C₄₂H₂₄O₁₄

CAS No.
SMILES

OC1=CC=C(C2(O3)C(C=C(C(O)=O)C=C4)=C4C3=O)C(OC5=C2C=CC(O)=C5)=C1.OC6=CC=C(C7(O8)C(C=CC(C(O)=O)=C9)=C9C8=O)C(OC%10=C7C=CC(O)=C%10)=C6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 41 mg/mL (54.48 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3287 mL 6.6434 mL 13.2867 mL
5 mM 0.2657 mL 1.3287 mL 2.6573 mL
10 mM 0.1329 mL 0.6643 mL 1.3287 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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Keywords:

5(6)-Carboxyfluorescein5(6)-FAM 5-(and-6)-Carboxyfluorescein mixed isomersOtherschimericpeptidesmembranetargetingfluoroprobetumourmappinglabelInhibitorinhibitorinhibit

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Product Name:
5(6)-Carboxyfluorescein
Cat. No.:
HY-15940
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