5(6)-Carboxyfluorescein
Based on 5 publication(s) in Google Scholar
5(6)-Carboxyfluorescein (5(6)-FAM) is an amine-reactive pH-sensitive green fluorescent probe. 5(6)-Carboxyfluorescein (5(6)-FAM) can be used to label proteins, peptides and nucleotides. 5(6)-Carboxyfluorescein can be used for the detection of tumour areas in vivo.
For research use only. We do not sell to patients.
- Purity: 99.66%
- CAS No.: 72088-94-9
- Formula: C42H24O14
- Molecular Weight:752.63
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 5(6)-Carboxyfluorescein
More
Biological Activity
5(6)-Carboxyfluorescein has two main characteristics: it has two wavelengths of maximum absorbance (465 and 490 nm) and its fluorescence emission (maximum, 515 nm) increases as a function of pH in the physiological pH range of 6-7.4[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CDF mice, bearing lymphoid leukemia P388[2]
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Dosage:5 mg/kg
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Administration:Injected intraperitoneally
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Result:Could be used for measurement and imaging of tumor tissue.
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Chemical Information
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CAS No. 72088-94-9
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Appearance Solid
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Molecular Weight 752.63
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Formula C42H24O14
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Color Yellow to orange
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SMILES
OC1=CC=C(C2(O3)C(C=C(C(O)=O)C=C4)=C4C3=O)C(OC5=C2C=CC(O)=C5)=C1.OC6=CC=C(C7(O8)C(C=CC(C(O)=O)=C9)=C9C8=O)C(OC%10=C7C=CC(O)=C%10)=C6
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Synonyms
5(6)-FAM; 5-(and-6)-Carboxyfluorescein mixed isomers
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (5)
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Journal Impact Factor
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Most Recent
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Nat Commun
2024 Oct 9;15(1):8748. PMID: 39384788 -
Nat Commun
2023 Jul 17;14(1):4261. PMID: 37460623 -
Environ Sci Technol
2025 Aug 19;59(32):17084-17091. PMID: 40767965 -
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Hypertension
Shear-Sensitive lncRNA AF131217.1 Inhibits Inflammation in HUVECs via Regulation of KLF4. [Abstract]2019 May;73(5):e25-e34. PMID: 30905197
Solvent & Solubility
DMSO : 58.33 mg/mL (77.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Maksim V Kvach, et al. 5(6)-carboxyfluorescein revisited: new protecting group, separation of isomers, and their spectral properties on oligonucleotides. Bioconjug Chem. Sep-Oct 2007;18(5):1691-6. [Content Brief]
[2]. Wen Ma, et al. A Cell Membrane-Targeting Self-Delivery Chimeric Peptide for Enhanced Photodynamic Therapy and In Situ Therapeutic Feedback. Adv Healthc Mater. 2020 Jan;9(1):e1901100. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3287 mL | 6.6434 mL | 13.2867 mL | 33.2169 mL |
| 5 mM | 0.2657 mL | 1.3287 mL | 2.6573 mL | 6.6434 mL | |
| 10 mM | 0.1329 mL | 0.6643 mL | 1.3287 mL | 3.3217 mL | |
| 15 mM | 0.0886 mL | 0.4429 mL | 0.8858 mL | 2.2145 mL | |
| 20 mM | 0.0664 mL | 0.3322 mL | 0.6643 mL | 1.6608 mL | |
| 25 mM | 0.0531 mL | 0.2657 mL | 0.5315 mL | 1.3287 mL | |
| 30 mM | 0.0443 mL | 0.2214 mL | 0.4429 mL | 1.1072 mL | |
| 40 mM | 0.0332 mL | 0.1661 mL | 0.3322 mL | 0.8304 mL | |
| 50 mM | 0.0266 mL | 0.1329 mL | 0.2657 mL | 0.6643 mL | |
| 60 mM | 0.0221 mL | 0.1107 mL | 0.2214 mL | 0.5536 mL |
5(6)-Carboxyfluorescein Related Classifications
HY-15940 Related Classifications
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